104株幽门螺杆菌插入序列IS605、IS606的分布  

Dissemination of insertion sequences IS605, IS606 among clinical isolates of Helicobacter pylori in China

作　　者：张茂俊[1] 张建中[1] 何利华[1] 郭浩岩[1] 尹焱[1] 周增芬[2] 

机构地区：[1]中国疾病预防控制中心传染病预防控制所腹泻室,北京102206 [2]昆明医学院第一附属医院消化科

出　　处：《中华流行病学杂志》2002年第5期366-369,共4页Chinese Journal of Epidemiology

基　　金：国家自然科学基金资助项目 (3 9870 0 3 2 ) ;国家高技术研究发展计划"863"计划资助项目 (2 0 0 1AA2 15 1610 2 )

摘　　要：目的　筛查中国 5地区幽门螺杆菌 (Hp)插入序列IS6 0 5、IS6 0 6的分布。方法　选取来自北京、福建、云南、沈阳、香港等地共 10 4株Hp ,用PCR及染色体打点杂交的方法筛查IS6 0 5、IS6 0 6的分布 ,并对其分布特点进行分析。结果　中国 5地区Hp菌株IS6 0 5的阳性率为 4 0 % ,IS6 0 6的阳性率为 18%。云南菌株IS6 0 5的阳性率有高于其他地区的趋势 ,不同地区IS6 0 6的分布差异无显著性。不同临床诊断结果分离的菌株IS6 0 5、IS6 0 6的分布差异无显著性。IS6 0 5的两个开码读框ORFA、ORFB共存。结论　中国 5地区Hp菌株广泛存在IS6 0 5、IS6 0 6 ,且IS6 0 5的分布可能与菌株分离个体的地理位置有关。Objective To study the distribution of IS605, IS606 among clinical isolates of Helicobacter pylori in China. Methods A total of 104 H.pylori strains isolated from 5 different geographic regions in China were analyzed by PCR and dot blot. Results Forty two strains out of the 104 isolates from 5 regions in China were found containing IS605 with 19 containing IS606. The frequency (66%) of IS605 positive strains from Yunnan province was higher than that from other areas. The different distribution of IS606 was neither associated with geographical regions nor with the presence of IS605 but IS606 were associated with the different clinical outcomes. However,the two reading frames ORFA and ORFB of IS605 were constantly coexisting. Conclusion In China, IS605 and IS606 of H. pylori were widely exisiting but the present of IS605 in H. pylori might be associated with geographic origin.

关 键 词：IS605 IS606 幽门螺杆菌 插入序列 IS 染色体打点杂交 分子流行病学 聚合酶链反应 

分 类 号：R346[医药卫生—基础医学]