一氧化氮在大鼠肝缺血预处理中的作用  

Effect of nitric oxide on liver ischemic precondition in rats

作　　者：詹勇强[1] 吕新生[1] 王志明[1] 李宜雄[1] 胡国璜[1] 曾庆华[1] 

机构地区：[1]中南大学湘雅医院普外科,湖南长沙410008

出　　处：《中国普通外科杂志》2002年第9期541-544,共4页China Journal of General Surgery

摘　　要：目的　探讨一氧化氮 (NO )在大鼠肝脏缺血预处理 (IP)中的作用。方法　 13 1大鼠随机分为缺血再灌注 (I/R)组、IP组及假手术 (S)组 ,观察术后 2h ,2 4h及 1周后血浆NO ,AST及ALT以及大鼠死亡率及肝脏组织病理改变。结果　 (1)血浆NOIP组在术后即升高 ,3个时段均明显高于S组 (P <0 .0 5 ,P<0 .0 1,P <0 .0 5 ) ;I/R组在 2h后低于S组 (P <0 .0 5 ) ,2 4h与S组无显著差异 (P >0 .0 5 ) ,1周时高于S组 (P <0 .0 5 ) ;IP组于 2h(P <0 .0 1)及 2 4h(P <0 .0 1)时均高于I/R组。 (2 )ALTIP组及I/R组在 2h(P <0 .0 5 )及 2 4h(P <0 .0 1)时均高于S组 ;IP组显著低于I/R组 (P <0 .0 5 ) ;1周时IP组与S组间差异无显著性 ,I/R组显著高于IP组 (P <0 .0 5 )及S组 (P <0 .0 1)。 (3 )IP组及I/R组血浆NO与ALT呈负相关 (P <0 .0 1,P <0 .0 5 )。 (4 )肝脏病理改变IP组较I/R组为轻。 (5 )术后 2h及累计动物死亡率I/R组显著高于S组及IP组 (P <0 .0 5 ) ,而IP组与S组无统计学差异。结论　IP对肝脏I/R具有保护作用 ,该作用可能与血浆NO升高有关 ,NO升高的峰值提前在经典IP中具有重要的作用。Objective To study the role of nitiric oxide (NO) in the liver ischemic precondition (IP) in rats. Methods 131 rats were randomly assigned to ischemia/reperfusion (I/R)group, IP group and sham-operation(S) group. The plasma NO, alanine aminotransferase (ALT) and aspartate aminotransferase (AST), the pathological change of liver and rat mortality were observed at 2 hours, 24 hours and 1 week after operation. Results (1) The plasma NO level grew soon after operation in group IP, and was significantly higher than that in group S in all three time points (P<0.05, P<0.01 and P<0.05, respectively.); but in group I/R,compared with group S, NO was significantly lower at 2h(P<0.05) , not significantly different at 24h(P>0.05), and higher at 1 week (P<0.05). In group IP,NO was higher than that in group I/R significantly at 2h (P<0.01)and 24h (P<0.01).(2)ALT activity in group IP was higher than that in group S at 2h (P<0.05) and 24h (P<0.01), but it was lower in group IP than that in group I/R (P<0.05) 1 week later, no statistical significance showed between group IP and group S, but in group I/R it was still higher than that in group IP (P<0.05) and group S (P<0.01).(3)The plasma NO level was in negative relation with ALT activity in group IP (P<0.01) and group I/R (P<0.05).(4)In group IP, the pathological changes were milder than those in group I/R.(5)In group I/R, the 2h and total mortality was significantly higher than that in group S and group IP(P<0.05,all), but there was no statistical difference between group IP and group S. Conclusions IP has a protective effect on liver I/R injury. This effect is relative to the increase of serum NO level. The peak level of NO coming earlier shows that NO plays an important role in the early protection of IP.

关 键 词：缺血预处理 一氧化氮 再灌注损伤 肝脏缺血 IP 

分 类 号：R657.3[医药卫生—外科学]