应重视幽门螺杆菌的分离培养  

The importance of separating culture in diagnosis of Helicobacter pylori infection

作　　者：王青[1] 汪丽霞[1] 史济经[2] 杨慧芳[3] 王洪涛[4] 张振华[4] 郭晓奎[4] 

机构地区：[1]上海市江湾医院检验科,上海200434 [2]上海市江湾医院内科,上海200434 [3]上海市江湾医院病理科,上海200434 [4]上海第二医科大学微生物教研室,上海200025

出　　处：《上海医学检验杂志》2002年第5期268-270,共3页Shanghai Journal of Medical Laboratory Sciences

摘　　要：目的　通过比较 3种不同幽门螺杆菌 (Helicobacterpylori,Hp)感染诊断方法之间的差异 ,使临床重视Hp的分离培养工作。 方法　对 2 0 0 0年我院胃镜检查者 312例同时作Hp分离培养、病理切片和快速脲酶试验 ,比较其检出率。结果　近期未用抗菌药物治疗的十二指肠溃疡患者活检材料中Hp分离培养检出率达93.75 % ,把其他 2种方法与分离培养相比 ,则快速脲酶试验存在 2 0 .72 %的假阳性和 3.98%的假阴性结果 ,病理切片存在 8.11%的假阳性和 32 .84%的假阴性结果 ;快速脲酶试验和病理切片组合后与分离培养相比 ,则存在 2 .99%的假阴性和 2 3.42 %的假阳性。结论　分离培养是Hp感染诊断的金标准 。Objective To demonstrate the imporatance of separating culture in the diagnosis of helicobacter pylori(Hp) Method Comparative determination of Hp by separating culture, pathological section and rapid urease test in 312 gastroscopy patients. Result The checking-out ratio of Hp separating culture in the biopsy materials of the duodenum ulcer patients reaches 93.75%. In the comparison of this result, the rapid urease test has 20.72% false positive and 3.98% false negative results, the patheological section has 8.11% false positive and 32.84% false negative results. The result of the combination of the rapid urease test and the pathological section has 2.99% false negative and 23.42% false positive. Conclusion The separating culture is the gold criterion for diagnosing Hp infection, which shall be applied in the clinical practice.

关 键 词：幽门螺杆菌 脲酶试验 病理切片 细菌株分离 细菌培养 

分 类 号：R446.5[医药卫生—诊断学] R378.99[医药卫生—临床医学]