酶联免疫方法检测粪便幽门螺杆菌抗原的评价  

Evaluation of the ELISA method for the determiuation of Helicobacter pylori antigen in feces

作　　者：伊妮[1] 乐建培[1] 吴瑞华[1] 王昕蕾[1] 

机构地区：[1]上海市静安区中心医院,上海200040

出　　处：《上海医学检验杂志》2002年第5期282-283,共2页Shanghai Journal of Medical Laboratory Sciences

摘　　要：目的　评价酶联免疫方法检测粪便幽门螺杆菌抗原 (Helicobacterpyloriantigen ,HpAg)的可靠性和准确性。方法　应用双抗体夹心酶联免疫吸附试验 (ELISA)检测Hp感染可疑者 140例的粪便标本中的HpAg、同时与其它 3种常用的临床诊断方法 [组织切片染色镜检、细菌分离培养 ,快速脲酶试验 (RapidUreaseTest,RUT) ]进行比较评价。结果　ELISA检测粪便HpAg阳性率 (84.3% )与其它 3种方法的阳性率差异无显著性。诊断敏感度和特异性分别为 98.3%和 83.3% ,阳性预期值 96 .6 % ,阴性预期值 90 .9% ,总有效率 95 .7%。结论　ELISA检测粪便HpAg感染简便易行 ,敏感性和特异性均较高 ,无创伤性 。Objective To evaluate the reliability of cliniical application of the ELISA method for the determination of hilicobacter pylori antigen (HpAg) in feces. Methods Comparative determination of clinical samples using ELISA method for feces HPAg, pathologic test of tissue slice, bacterial culture and rapid urease test (RUT). Results The positive rate of ELISA for feces HPAg was 84.3%, no significant difference with other 3 mothods. The diagnosfie sensitivity and specificity of ELISA method were 98.3% and 83.3% respectively. Conclusion The ELISA for feces HPAg is a valuable test for screening Hp infection.

关 键 词：酶联免疫吸附试验 粪便检测 幽门螺杆菌 抗原 

分 类 号：R446.62[医药卫生—诊断学]