中药外敷对哮喘豚鼠肺组织β和M受体含量的影响  

Effects of Herbal Penetration Therapy on βAdrenergic Receptor and Muscarinic Receptor Expression in the Lung Tissue of Guinea Pigs with Experimental Allergic Asthma

作　　者：闵亮[1] 沈惠风[1] 李鹤[1] 

机构地区：[1]上海第二医科大学附属仁济医院中医科,上海200001

出　　处：《湖南中医学院学报》2002年第3期22-24,共3页Journal of Hunan College of Traditional Chinese Medicine

基　　金：上海市科学技术发展基金资助项目(984019029)

摘　　要：目的观察豚鼠哮喘急性发作时肺组织β肾上腺素能受体和M胆碱能受体含量变化及中药外敷对其影响。方法用Hutson法造模和诱喘 ,治疗组予中药外敷 ,对照组予 0 .9%生理盐水浸润纱布外敷 ,2周后取肺组织 ,用放射配基结合分析法测定其中β受体和M受体含量。 结果豚鼠哮喘急性发作时肺组织β受体含量明显降低 ,M受体含量明显升高 ,而中药外敷能使其恢复正常。结论提高肺组织 β受体含量和降低M受体含量是中药外敷治疗哮喘的机理之一。?Objective To observe the changes of β adrenergic receptor and muscarinic receptor4 expression in the lung tissue of guinea pigs with experimental allergic asthma, when they were challenged, and the therapeutic effects of herbal penetration therapy on their expression. Methods The asthma model was induced by Huston's method among guinea pigs and herbs were externally applied for 30min q.o.d.. A control group of guinea pigs was applied with wet gauzes in 9g/L normal saline in the same way. They were killed after two weeks. The β adrenergic receptor and muscarinic receptor expression in lung tissues was observed by radioligand binding assay. Results The β adrenergic receptor expression was down regulated and the muscarinic receptor expression was up regulated, when asthma was challenged. In contrast,they were up regulated and down regulated respectively, when given with herbal penetration therapy. Conclusion One of the important mechanisms of herbal penetration therapy to treat allergic asthma is to up regulate the expression of β adrenergic receptor and to down regulate that of muscarinic receptor in lung tissues. 〔

关 键 词：哮喘 中药外敷 Β肾上腺素能受体 M胆碱能受体 细辛 白芥子 

分 类 号：R285.5[医药卫生—中药学] R259.622.5[医药卫生—中医学]