槐耳清膏对耐顺铂人肺腺癌细胞系A_(549)^(DDP)逆转的实验研究  

Reversion of PS-T on Cisplatin Resistance of Human Lung Adenocarcinoma Cellline A_(549)^(DDP) in Vitro

作　　者：黄涛[1] 孔庆志[1] 卢宏达[1] 戴观书[1] 

机构地区：[1]武汉市第四医院暨华中科技大学同济医学院附属普爱医院,武汉430033

出　　处：《中国药师》2002年第9期517-518,521,共3页China Pharmacist

基　　金：武汉市卫生局科研基金(2000-148)

摘　　要：目的:探讨槐耳清膏对耐顺铂的人肺腺癌细胞A_(549)^(DDP)化疗敏感性的逆转作用及其效能。方法:以体外培养的对耐顺铂的人肺腺癌细胞A_(549)^(DDP)为实验模型,运用MTT法研究经不同浓度的槐耳清膏作用后,该模型所需顺铂IC_(50)的变化,并运用流式细胞仪研究相同作用条件下A_(549)及A_(549)^(DDP)的凋亡发生率。结果:槐耳清膏对A_(549)和A_(549)^(DDP)的细胞毒性作用相似,并可致凋亡,其IC_(50)分别为(1.84±0.52)mg·ml^(-1)和(2.01±0.44)mg·ml^(-1)两者无显著性差异(P>0.05)。经槐耳清膏作用后A_(549)及A_(549)^(DDP)的顺铂IC_(50)均呈下降趋势,并与槐耳清膏有剂量依赖性。给予1mg·ml^(-1)槐耳清膏作用40h后再经相应的顺铂作用的A_(549)及A_(549)^(DDP)细胞凋亡的发生率无显著性差异(P>0.05);但较同剂量的顺铂单独作用于A_(549)及A_(549)^(DDP)凋亡率有极显著差异(P<0.01)。结论:槐耳清膏除自身有凋亡作用外,亦可以逆转人肺腺癌细胞对顺铂的耐药作用,提高顺铂对A_(549)^(DDP)化疗的敏感性。Objective: To investigate the possibility and efficacy of polysaccharide PS-T on reversion of reversion of cisplatin-resistance in human lung adenocarcinoma cellline A549DDP. Method: A model of Cellline A549DDP of cisplatin resistance in -vitro was built. Exposed in different concentration. The IC50s of cisplatin of A549 and A549DDP were evaluated by MTT assay, the apoptotic index measured by flow cytometry in the same condition. Results:There was a resemblance in the cytotoxic effect of PS-T on A549 and A549DDP when the PS-T concentration was increased gradually, and the IQos of PS-T on A549 and A549DDP were (1.84?

关 键 词：实验研究 槐耳清膏 人肺腺癌细胞系A549 顺铂 耐药性 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学] R273.42[医药卫生—中医学]