药用野生稻(Oryza officinalis Wall)和转bar基因水稻(Oryza sativa L.)花粉杂交的基因漂移  

Gene flow of pollen cross between Oryza officinalis Wall and transgenic rice with bar gene

作　　者：宋小玲[1] 强胜[1] 刘琳莉[1] 徐言宏[1] 刘友良[1] 

机构地区：[1]南京农业大学杂草研究室,江苏南京210095

出　　处：《南京农业大学学报》2002年第3期5-8,共4页Journal of Nanjing Agricultural University

基　　金：国家科技部转基因专项基金资助 (E2 0 0 10 2 )

摘　　要：采用生殖生物学方法研究了药用野生稻和转bar基因水稻花粉杂交的基因漂移。结果表明 ,供试水稻花粉在药用野生稻柱头上的萌发生长与药用野生稻自花授粉花粉的萌发生长有一定差异 ,表现在穿过柱头的花粉粒百分率及内容物释放和正在凝缩、释放的花粉粒百分率较少。虽然转基因水稻花粉能在药用野生稻柱头上正常萌发生长 ,并能释放内容物 ,但杂交后结实率为 0 ,表明转基因水稻和药用野生稻杂交不亲和。他们的不亲和性不是在花粉的萌发和穿过柱头这一阶段 ,具体原因有待进一步研究。在本实验条件下转基因水稻和药用野生稻没有发生成功的基因交流。The gene flow of pollen cross between wild rice(O.officinalis Wall)(female)and two varieties of transgenic rice with bar gene(Y0003 and 99 t)(male)was studied by means of reproductive biology.The results showed that the pollen grains of two varieties of transgenic rice could germinate on the stigmas of wild rice and their pollen tubes could grow and penetrate stigmas of wild rice normally,but the pollen germination and growth of cross differed from that of self pollination of wild rice,representing that the percent of pollen grains penetrated wild rice stigmas or condensed and being condensing or releasing pollen inclusions were lower than that of self pollination.The emasculated wild rice pollinated by transgenic rice pollen grains couldn′t seed.From above results,It could be concluded that wild rice and transgenic rice were sexual incompatible,and the sexual incompatibility didn′t display on the stage of transgenic rice pollen grains germination and pollen tube penetrating the wild rice stigmas.The reason need to be studied further.Under this experiment condition,gene flow between wild rice and transgenic rice with bar gene didn′t occur successfully.

关 键 词：药用野生稻 转BAR基因水稻 花粉杂交 基因漂移 生殖生物学 抗除草剂基因 安全性 

分 类 号：S451.2[农业科学—植物保护] S511