腰带长体茧蜂寄生后亚洲玉米螟体内抗氧化酶活性及组织特异性  

The activity and tissue specificity of antioxidant enzymes in larvae of Ostrinia furnacalis(Guene)parasitized by Macrocentrus cingulum Briscke

作　　者：冯从经[1] 戴华国[2] 符文俊[1] 

机构地区：[1]中国科学院上海植物生理生态研究所,上海200025 [2]南京农业大学农业部病虫监测与治理重点开放实验室,江苏南京210095

出　　处：《南京农业大学学报》2002年第3期31-35,共5页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金重点项目资助 (3 993 0 0 3 0 )

摘　　要：测定被腰带长体茧蜂寄生的亚洲玉米螟 5龄幼虫体内不同组织中超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)、过氧化物酶 (POD)活性 ,以研究寄主昆虫免疫过程中产生的活性氧自由基 (ROS)与抗氧化酶的关系。结果表明 :被寄生后 ,寄主幼虫体内应激产生大量ROS ,从而诱导SOD、CAT、POD活性明显上升 (P <0 0 1)。不同组织中SOD、CAT和POD的分布存在差异 ,说明亚洲玉米螟被腰带长体茧蜂寄生所诱导产生的抗氧化酶可协同作用 ,从而清除体内产生的过量ROS。这种组织特异性有利于保存寄主 ,使寄生蜂成功寄生。The activity of antioxidant enzymes,including superoxide dismutase(SOD),catalase(CAT)and peroxidase(POD),was determined in vivo in different tissues of 5th instar larvae of O.furnacalis parasitized by M.cingulum.The results showed that a great deal of reactive oxygen species(ROS)were generated in the host larvae,and the increase of SOD activity was induced.In addition,the distribution of SOD,CAT and POD in the hemolymph,integument and fat body differed from each other.Thus,ROS induced in vivo in different tissues of 5th instar larvae of O.furnacalis parasitized by M.cingulum could be scavenged by the antioxidant enzymes,which was benefit for the sucessful development of M.cingulum in the host larvae.

关 键 词：组织特异性 亚洲玉米螟 腰带长体茧蜂 活性氧自由基 抗氧化酶 酶活性 寄生蜂 天敌 

分 类 号：S435.132[农业科学—农业昆虫与害虫防治]