RAPD法分析鉴定大白菜的亲缘关系  

To Analyze and Determine the Relationship Among Chinese Cabbages by RAPD Method

作　　者：曲士松[1] 黄宝勇[1] 石琰[2] 孙晋斌[1] 张杰[1] 徐培文[1] 

机构地区：[1]山东省农科院蔬菜研究所,山东济南250100 [2]山东农业大学

出　　处：《莱阳农学院学报》2002年第2期108-111,共4页Journal of Laiyang Agricultural College

摘　　要：以 2 0份不同抱球方式的大白菜品种资源为材料 ,用 2个随机引物进行了RAPD分析。结果表明 :11个品种具有明显的多态性差异。叠抱类型的大白菜品种间的相似系数在 2 .6 4 5 8～ 3.2 395之间 ,平均为 2 .94 2 6 ;拧抱类型的大白菜品种间的相似系数在 1.732 1～ 4 .930 8之间 ,平均为 3.0 2 0 4 ;合抱类型的大白菜品种间的相似系数在1.732 1～ 3.0 0 0 0之间 ,平均为 2 .36 6 0。这些差异与不同白菜品种的来源地及定向培育有关。研究结果对白菜远缘杂交育种具有一定的参考价值。Using 20 germplasm resources of different leaf ball types of Chinese cabbage as materials,carried on RAPD analysis by using two random primers. The result indicated that there were obvious pleiomorphism difference among 11 varities. Repeat type among Chinese cabbage varities had similar coefficient bettwen 2.6458～3.2395, mean is 2.9426; twist type among Chinese cabbage varities had similar coefficient bettwen 1.7321～4.9308, mean is 3.0204; Close type among Chinese cabbage varities had similar coefficient bettwen 1.7321～3.0000, mean is 2.3660; this difference is relative with the origin and cultivate directional of different Chinese cabbage varities. This result is of great reference value to distant hybridization of Chinese cabbage.

关 键 词：鉴定 亲缘关系 大白菜 分子标记 抱球方式 RAPD 植物学性状 

分 类 号：S634.1[农业科学—蔬菜学]