10-羟基喜树碱对异抗原刺激下大鼠淋巴细胞增殖的抑制作用  

IN VITRO STUDY OF THE INHIBITORY EFFECT OF HCPT ON RAT LYMPHOCYTE PROLIFERATION STIMULATED BY ALLOANTIGEN

作　　者：蔡明[1] 石炳毅[1] 莫春柏[1] 钱叶勇[1] 王燕[1] 周文强[1] 

机构地区：[1]解放军第309医院,北京100091

出　　处：《解放军医学杂志》2002年第10期860-861,共2页Medical Journal of Chinese People's Liberation Army

基　　金：全军医学科研"十五"计划重大项目基金资助课题(编号01Z097)

摘　　要：为探讨10-羟基喜树碱（HCPT）对异基因大鼠器官移植物急性排斥反应抑制作用的机制和有效浓度,制作器官移植体外动物模型,以SD大鼠淋巴细胞为刺激物,Wistar大鼠淋巴细胞为反应物行体外混合淋巴细胞培养试验,观察HCPT对淋巴细胞增殖反应的抑制作用,以及不同剂量浓度与抑制效果之间的量-效关系。结果显示,100μg/ml、10μg/ml和2μg/ml浓度的HCPT对SD大鼠淋巴细胞刺激下Wistar大鼠淋巴细胞的增殖均有明显的抑制作用,抑制指数分别为0.734±0.085,0.537±0.361,0.503±0.225;100μg/ml组的抑制效果明显高于10μg/ml和2μg/ml组（P<0.05,P<0.01）。提示HCPT对抗原刺激下大鼠淋巴细胞的分化增殖具有明显的抑制作用,且抑制效果与药物浓度具有显著的相关性。To study the mechanism of inhibition and the effective concentration of HCPT in treatment of acute allgraft rejection, we made an in vitro model using mixed lymphocyte culture (MLC) with the stimulator lymphocytes from SD rats and the responder ones from Wistar rats. We observed the results of the inhibitory effect of HCPT on the reaction of the lymphocytic proliferation as well as the dose-effect relationship of HCPT. The results showed that HCPT at concentrations of 100μg/ml, 10μg/ml and 2μg/ml inhibited the proliferative reaction significantly, the inhibition index were 0. 734 ± 0. 085, 0. 537±0. 361 and 0. 503 ± 0. 225, respectively. The efficacy of 100μg/ml HCPT was significantly higher that of than that of both 10μg/ml (P<0. 005) and 2μg/ml (P<0. 01). Our conclusion is that HCPT inhibits proliferative reaction of the rat lymphocytes stimulated by alloantigen and the efficacy of the inhibition depends significantly on the concentration of HCPT.

关 键 词：器官移植 免疫抑制剂 细胞分化 排斥反应 移植免疫学 羟基喜树碱 HCPT 

分 类 号：R392.4[医药卫生—免疫学] R617[医药卫生—基础医学]