肾移植受者外周血B淋巴细胞增殖活化与CD11a和CD54表达的关系  

ANALYSIS ON THE RELATIONSHIP BETWEEN PROLIFERATION AND ACTIVATION OF B LYMPHOCYTES AND CD11a, CD54 EXPRESSION IN RENAL ALLOGRAFT RECIPIENTS

作　　者：蒋立城[1] 潘光辉[2] 丁红[3] 白寒[3] 裘宇容[3] 

机构地区：[1]解放军第91医院,兖州272100 [2]广州医学院第二附属医院 [3]第一军医大学南方医院

出　　处：《解放军医学杂志》2002年第10期874-875,共2页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金资助课题(编号39570200)

摘　　要：为探讨肾移植受者B淋巴细胞活化与CD11a和CD54表达的关系,作者采用流式细胞仪-单克隆抗体荧光免疫技术动态观测10例肾移植稳定病人和10例排斥反应病人外周血淋巴细胞 CD19、CD11a和CD54的表达。结果显示,术后3天时两组CD19、CD54表达明显高于术前,且排斥前CD19表达明显高于同期稳定组;排斥反应时CD11a由（31.9±12.4）％升至（49.5±20.2）％（P<0.01）,抗排斥治疗后则迅速下降。稳定组CD54与CD19的表达变化呈正相关（r=0.7951,P<0.05）,排斥组CD19的表达变化较CD11a提前2～4天（γ=0.9806,P<0.05）。研究表明,B细胞的增殖活化可能与CD54的表达增加有关,监测CD11a和CD19的表达变化分别有助于早期急性排斥反应的诊断和早期急性排斥反应的早期诊断。To investigate the correlation between activation of B lymphocytes and CD11a, CD54 expression in renal allograft recipients, the dynamic changes in CD19, CD11a and CD54 levels in lymphocytes at different time points were examined by using mAb-FACS-immunofluores-cence technique in 10 renal allograft recipients in stable course(stable group) and 10 with acute rejection(rejection group) after operation. The expression levels of CD19 and CD54 in lymphocytes were significant higher than those of the pre-operation levels in both stable group and rejection group on the 3rd day after kidney transplantation, and the CD19 expression level was significant higher before rejection in the rejection group than that in stable group at the same time points; The expression of CDlla increased from (31. 9 ±12. 4)% to (49. 5 ± 20. 2)% during rejection and decreased significantly after anti-rejection treatment. A negative correlation was found between CD54 and CD19 expression in the stable group(r=0. 7951, P<0. 05), and the change in CD19 expression was 2 to 4 days earlier than that of CD11a in the rejection group(r= 0. 9806, P<0. 05). Therefore, proliferation and activation of B lymphocyte may be correlated to increase of lymphocyte CD54 expression, and dynamic investigation of CD11a and CD19 expression levels in lymphocytes may be helpful for diagnosis of acute rejection at early time after kidney transplantation.

关 键 词：肾移植 B淋巴细胞 增殖 活化 细胞粘附分子 移植免疫学 流式细胞仪-单克隆抗体荧光免疫技术 

分 类 号：R699.2[医药卫生—泌尿科学] R392.4[医药卫生—外科学]