大鼠胃窦粘膜G、D细胞的免疫电镜观察  

IMMUNOELECTRON MICROSCOPIC STUDY ON G CELLS AND D CELLS IN ANTRAL MUCOSA OF RAT

作　　者：孙凤蓬[1] 宋于刚[1] 覃汉荣[2] 肖莎[2] 余力[2] 赵彤[3] 

机构地区：[1]第一军医大学南方医院,广州510515 [2]第一军医大学珠江医院 [3]第一军医大学病理学教研室

出　　处：《解放军医学杂志》2002年第10期893-894,共2页Medical Journal of Chinese People's Liberation Army

基　　金：广东省自然科学基金资助课题(编号010578);广东省重点科技攻关项目(编号99-13)

摘　　要：采用胶体金免疫电镜方法并加以改良,对大鼠胃窦粘膜G、D细胞进行观察,同时对G、D细胞内胶体金颗粒数目进行定量分析。结果发现,免疫胶体金颗粒分布于G、D细胞中,标记胃泌素或生长抑素的金颗粒主要呈分叶状或岛状的聚集体,多数游离存在于G、D细胞基底膜侧的胞浆中,少部分定位在G、D细胞核内;G细胞中金颗粒聚集体的金颗粒数目为107.04±19.68,D细胞中金颗粒聚集体的金颗粒数目为83.36±17.58。Revised immunoelectron microscopic technique was used to examine G cells and D cells in rat antral mucosa. The number of colloidal gold granules in G and D cells was analysed through quantitative analysis. The results showed that immunological granules of colloidal gold distributed in both G and D cells. Gastrin- or somatostatin-labeled granules were present mainly as lobation-like or island-like congeries. Most of the golden congeries dissociated in the cytoplasm of G or D cells, being in the basement membrane side. A few of the golden congeries located in nucleus. The number of golden granules was 107. 04 ±19. 68 and 83. 36±17. 58 per one G and D cell, respectively.

关 键 词：胃粘膜 G细胞 D细胞 免疫电镜 定量分析 

分 类 号：R333.2[医药卫生—人体生理学]