瘢痕疙瘩周围皮肤成纤维细胞凋亡的实验研究  

STUDY ON APOPTOSIS OF FffiROBLASTS DERIVED FROM THE SURROUNDING SKIN OF KELOIDS

作　　者：段红杰[1] 沈光裕[1,2] 高建华 沈光裕[1,2] 刘真喜[2] 

机构地区：[1]解放军第187医院,海口571159 [2]第一军医大学南方医院

出　　处：《解放军医学杂志》2002年第10期908-910,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金资助课题(编号39870807)

摘　　要：探讨瘢痕疙瘩周围皮肤是否存在异常成纤维细胞,以期进一步了解瘢痕疙瘩的发展机制。体外培养瘢痕疙瘩及其周围皮肤成纤维细胞,运用流式细胞仪比较无血清培养条件下和FasMcAb作用下瘢痕疙瘩及其周围皮肤成纤维细胞凋亡率的差异。结果显示,无血清培养条件下瘢痕疙瘩周围皮肤（距瘢痕疙瘩边缘0.5cm范围内）成纤维细胞凋亡增长率介于瘢痕疙瘩和正常皮肤之间。FasMcAb作用下,瘢痕疙瘩周围皮肤成纤维细胞凋亡率增加,与正常皮肤比较差异有显著性意义（P<0.01）,但总体上与瘢痕疙瘩差异无显著性意义（P>0.05）。研究表明,瘢痕疙瘩周围皮肤至少有部分成纤维细胞不能正常凋亡。The aim of this study was to investigate if there are abnormal fibroblasts derived from the skin surrounding keloids in order to have a better understanding for keloid progression. All the samples were used for cell culture. Flow cytometry was used to compare the apoptotic rate of fibroblasts derived from keloid and its surrounding skin, when it was cultured in serum-deprived medium for 24 hours or was induced by Fas antibody. After cultured in serum-deprived medium for 24 hours, the apoptotic rate of fibroblasts derived from the surrounding skin of keloid increased to an amount between that of normal skin and keloids. The apoptotic rate of normal skin fibroblasts increased more than that of keloids. Moreover, when induced by Fas antibody, the apoptotic rate of fibroblasts derived from the surrounding skin increased not so high as that of normal skin(P<0. 01). In general, there were no significant differences of cell apoptotic rates between keloids and its surrounding skin(P>0. 05). Therefore, at least there are some fibroblasts in the surrounding skin of keloids, in which apoptosis can not be induced as in normal skin.

关 键 词：细胞凋亡 实验研究 瘢痕疙瘩 周围皮肤 成纤维细胞 脱噬作用 

分 类 号：R619.6[医药卫生—外科学]