血性脑脊液对内皮功能影响及药物干预的研究  

Effect of BCSF on the secretion of endothelin and nitric oxide in endothelial cells and pharmacological intervention

作　　者：邵国富[1] 包仕尧[1] 张志琳[1] 楚冰 

机构地区：[1]苏州医学院附属第二医院,江苏苏州215004

出　　处：《中国组织工程研究》2000年第14期34-35,共2页Chinese Journal of Tissue Engineering Research

摘　　要：目的 观察血性脑脊液（ＢＣＳＦ）对内皮细胞（ＥＣ）分泌内皮素（ＥＴ）和一氧化氮（ＮＯ）的影响及川芎嗪（ＬＺ）、尼莫地平（ＮＭ）的干预作用。方法建立脐静脉ＥＣ体外培养的方法，测定ＥＴ、ＮＯ的代谢产物ＮＯ_Ｘ－ 含量，并观察ＢＣＳＦ和ＮＭ及ＬＺ的作用。结果ＬＺ和ＮＭ可抑制正常体外培养的ＥＣ分泌ＥＴ，对ＮＯ无影响；不同时间ＢＣＳＦ均使ＥＴ分泌增加，ＮＯ分泌减少；ＬＺ既能减少ＢＣＳＦ引起的ＥＴ升高，又可抑制ＮＯ的下降，而ＮＭ仅能抑制ＥＴ的增高，对ＮＯ无明显影响。结论蛛网膜下腔出血（ＳＡＨ）后，通过破坏ＥＴ／ＮＯ的平衡，导致脑血管痉挛（ＣＶＳ）。ＬＺ和ＮＭ可通过抑制这一作用而防治ＣＶＳ。Objective To study the effect of BCSF on the ET and NO from human EC and pharmacologial intervention of LZ and NM. Method The EC cultures from human unbilical vein were established. Concentration of ET and NO were determined using radioimmunoassy and HNO₃ reductase respectively and effects of BCSF, LZ and NM were conducted. Result ET secretion farm nomal EC could be inhibited by LZ and NM wheras NO yield was not affected. ET elevaton induced by BCSF could be inhibited by LZ as well as NM while NO reduction could only be inhibited by LZ. Conclusion LZ and NM could be used in prevention and treatment CVS following subarachnoid hemorrage（SAH） by inhibiting ET elevation and NO reduction.

关 键 词：血性脑脊液 内皮素 一氧化氮 脑血管痉挛 川芎嗪 尼莫地平 

分 类 号：R4[医药卫生—临床医学]