胚胎来源的同种异体组织工程化软骨形成的研究  

An experiment study on fetal allogenic tissue engineered cartilagr

作　　者：夏万尧[1] 曹谊林[1] 商庆新[1] 刘彦春[1] 王健[1] 崔磊[1] 

机构地区：[1]上海第二医科大学附属第九人民医院,上海200011

出　　处：《中国组织工程研究》2001年第9期57-58,共2页Chinese Journal of Tissue Engineering Research

基　　金：上海市科学技术委员会基金资助项目!(98JC14026)

摘　　要：目的研究探讨同种异体组织工程化软骨在动物体内形成。方法取怀孕 100 d胚胎羊的关节软骨经体外制成细胞生物材料复合物，并注射到异体羊腹壁皮下，每隔 2周取材，连续半年，对所取标本进行评价。结果在同种异体动物体内能形成软骨组织并与正常软骨组织有相似的组织学特性。第 2、 4周的软骨组织有一定程度的免疫排斥反应和炎症细胞浸润。第 6周免疫反应减轻。结论应用组织工程技术可在同种异体动物体内再生软骨组织，再生的组织周围存在一定程度的免疫反应，随接种时间的延长而逐渐减弱。Objective To investigate the formation of engineered cartilage in vivo with allogenic chondrocytes.Methods Joint cartilage from ram embryos(Pregnancy:100days)was mixed complex subcantenously.Collect the novel tissue every two weeks along for six months were evaluate the characteristics of novel tissue.Result Novel cartlage can regenerate in allograft animals,with similar histological properties of chondrocytes,aminoglycan and collagen distribution to normal cartilage.The novel cartilage observed histologically showed apparent surrounding inflammatory cells in two and four week and the tissue of cartilage came to mature since the sixth week,with less inflammatory reaction,but not disappeared.Conclusion Novel cartilage can generate in allograft animals with tissue engineering approach,with certain immunoreaction surrounding.Such reaction will be weakened gradually as time.

关 键 词：软骨组织 同种异体 组织工程 

分 类 号：R4[医药卫生—临床医学]