RP-HPLC梯度法测定血塞通滴丸中三七皂苷R_1、人参皂苷Rb_1及人参皂苷Rg_1的含量  

Determination of notoginsenoside R_1 and ginsenoside Rg_1, Rb_1 in Xuesaitong Dripping Pills by RP-HPLC gradient elution method

作　　者：王梅[1] 范亚刚[1] 

机构地区：[1]云南省药品检验所,云南昆明650011

出　　处：《中成药》2002年第9期681-683,共3页Chinese Traditional Patent Medicine

摘　　要：目的 :用高效液相色谱梯度洗脱法同时检测血塞通滴丸中三七皂苷R1、人参皂苷Rb1、人参皂苷Rg13种皂苷含量的方法 ,为制定质量标准中含量测定方法及含量限度提供了依据。方法 :用大连SpherisorbC18分析柱 ,乙腈 水线性梯度洗脱 ,0～ 15min(2 0∶80 - 4 0∶6 0 ) ,流速 1.0mL·min-1,检测波长 2 0 3nm。结果 :R1,Rf1和Rb1线性范围分别为 1.0 2～ 9.18μg ,4 .8～ 4 3.4 μg和 4 .6～ 4 1.6 μg。该方法回收率R1为 10 1.0 % (RSD =3.18% ) ,Rb1为 10 0 .0 % (RSD =1.19% ) ,Rg1为 99.5 % (RSD =3.0 2 % )。结论 :HPLC梯度洗脱法能将多种皂苷很好地分离检测 ,提高了时效 ,减少了误差 ,结果表明该方法准确可靠 ,重现性好 。Objective: A new HPLC gradient elution was studied for the determination of notoginsenoside R 1, ginsenoside Rg 1 and Rb 1 in Xuesaitong Dripping Pills. Methods:C 18 column was used with a mobile phase of acetonitrile water 0～15 min. of (20∶80～40∶60) gradient elution, post time was 5 min, the wavelength of detecter was set at 203nm. Results: The linear range of R 1, Rg 1 and Rb 1 was 1.02～9.18μg,4.8～ 43.4μg and 4.6～41.6μg, respectively, the average recovery rate was 101.0% ( RSD =3.18%), 99.5%( RSD =3.02%) and 100.0%( RSD =1.19), respectively. Conclusion: The method is rapid, accurate, sensitive and relieble. The results show that the method can be used to control quality of products.

关 键 词：RP-HPLC梯度洗脱酸法 三七皂甙R1 人参皂甙RB1 人参皂甙RG1 血塞通滴丸 含量测定 

分 类 号：R286.0[医药卫生—中药学]