云芝糖肽的HPCE分析方法的建立  

Establishment of method for the analysis of Yun Zhi Polysaccharopeptide(PSP) by HPCE

作　　者：袁萍[1] 洪筱坤[2] 

机构地区：[1]上海师范大学,上海200234 [2]上海中医药大学,上海200032

出　　处：《中成药》2002年第9期698-700,共3页Chinese Traditional Patent Medicine

摘　　要：目的 :为建立云芝糖肽 (PSP)高效毛细管电泳 (HPCE)的分析方法从而建立PSP的特征指纹谱而进行的电泳条件优化。方法 :采用高效毛细管电泳法 (CZE) ,通过对电解质种类、浓度、电解质溶液的 pH、电压、温度以及毛细管柱长和柱径等参数的优化 ,对PSP(糖蛋白 )和作为标准品的牛血清白蛋白 (BSA)进行分离分析。结果 :10mmol·L-1的硼酸缓冲液 ,pH =9.18,毛细管直径 5 0 μm ,柱长 5 7cm ,有效柱长 4 8.5cm的泡状柱 ,运行电压 30KV ,温度 2 5 °C ,样品用 5 0mbar进样8S ,分析时间 7min ,DAD全波长扫描检测条件下可以成功地进行PSP和BSA的分离分析。结论 :本法简便 ,快速 ,稳定 。Objective: To establish the analytical method for the characteristic electropherograms of PSP by High Performance Capillary Electrophoresis(Capillary Zone Electrophoresis). Method: Types, concentration and pH of electrolyte, voltage, temperature, length and size of column were investigated for the analysis of PSP and BSA which is as standard using capillary zone electrophoresis. Results: The optimum condition is that 10mmol·L -1 boric acid buffer, pH9.18, a column 50μm×57cm(HP, effective length of 48.5cm), 30KV, temperature 25 °C ,DAD detection. The analysis is ended within 7min. Conclusion: The method is simple, rapid and reliable. The characteristic electropherograms of PSP can be established.

关 键 词：云芝糖肽 PSP 高效毛细管电泳 CZE DAD检测 特征指纹谱 中药 

分 类 号：R284.1[医药卫生—中药学]