单颗粒冷冻电镜技术在核糖体抗生素耐药机理研究中的应用  

Prospect of single-particle cryo-electron microscopy in revealing mechanisms of ribosomal antibiotics resistance

作　　者：王婧芬 华孝挺[2] 张小东 吴航军 张兴[1,2,4] 俞云松 吴永平[3] WANG Jing-Fen;HUA Xiao-ting;ZHANG Xiao-dong;WU Hang-jun;ZHANG Xing;YU Yun-song;WU Yong-ping(Department of Cell Biology,Zhejiang University School of Medicine,Hangzhou Zhejiang 310058;Sir Run Run Shaw Hospital,Zhejiang University School of Medicine,Hangzhou Zhejiang 310058;College of Animal Science and Technology,Zhejiang A&F University,Linan Zhejiang 311300;Center of Cryo-Electron Macroscopy,Zhejiang University,Hangzhou Zhejiang 310058,China)

机构地区：[1]浙江大学基础医学院细胞生物学系,浙江杭州310058 [2]浙江大学医学院附属邵逸夫医院,浙江杭州310058 [3]浙江农林大学动物科技学院,浙江临安311300 [4]浙江大学冷冻电镜中心,医学院附属邵逸夫医院电镜诊断中心,浙江杭州310058

出　　处：《电子显微学报》2019年第5期550-555,共6页Journal of Chinese Electron Microscopy Society

基　　金：中央高校基本科研业务费项目校长专项基金(No.2018XZZX001-13);科技部重大专项(Nos.2018YFA0507700,2017YFA0504803);浙江省自然科学基金资助项目(No.LQ17C050001)

摘　　要：抗生素是现代医学的基础,随着抗生素在临床上广泛长期应用,细菌的耐药性日益显著,已成为人类健康的威胁。核糖体负责翻译遗传信息合成细胞中的所有蛋白质,是抗生素的主要靶点之一。本文从显微结构层次对核糖体抗生素的耐药性机理进行了总结,论述了核糖体基因突变,核糖体修饰及核糖体的保护机制导致细菌耐药性产生的分子机理,展望了未来冷冻电镜显微技术在揭示核糖体抗生素耐药性机理研究中的作用和前景。Antibiotics are the foundation of modern medicine,but with the widespread use of antibiotics in clinical practice,bacterial resistance has increasingly become prominent and a global health problem.Ribosome synthesizes proteins using genetic information,and is one of the main targets of antibiotics.This paper summarizes the structural bases of ribosomal antibiotics resistance,discusses the molecular mechanisms of ribosomal gene mutation,modification and protection that lead to bacterial resistance,and looks forward to the roles and prospects of single-particle cryo-electron microscopy(cryo-EM)in further revealing mechanisms of ribosomal antibiotics resistance.

关 键 词：冷冻电镜 抗生素 细菌耐药性 核糖体 核糖体基因突变 

分 类 号：R978.1[医药卫生—药品]