阿斯匹林铜的高效液相色谱分析  

Determination of Copper Aspirinate by High Performance Liquid Chromatography

作　　者：徐友志[1] 尹笃林[1] 伏再辉[1] 杨明生[1] 

机构地区：[1]湖南师范大学化学化工学院化学系,中国长沙410081

出　　处：《湖南师范大学自然科学学报》2002年第3期56-58,共3页Journal of Natural Science of Hunan Normal University

基　　金：国家自然科学基金资助项目 (2 97730 1 3);湖南省自然科学基金资助项目 ([2 0 0 0 ] 2 37 00JJY2 0 0 1 )

摘　　要：建立了用于新药阿斯匹林铜的高效液相色谱分析方法 .采用YWG C1 8柱 ,在室温下 ,n(乙腈 )∶n(水 )(内含 0 .2 %三乙胺 ,用H3 PO4调pH值至 2 ) =32∶68为流动相 ,药用标准品阿斯匹林和水杨酸为对照品 ,在紫外 2 30nm处检测阿斯匹林和水杨酸的含量 ;阿斯匹林铜和水杨酸铜的最小检测限为 0 .0 52mg·L-1 ,0 .0 4mg·L-1 ;阿斯匹林铜和水杨酸铜的平均回收率为 99.7% ,99.9% .A method for determining copper aspirinate Cu2 (ASP 4 has been studied by using reversed-phase high performance liquid chromatography with YWG-C18 column, n (MeCN) : n (H2O) (containing 0.2 % triethylamine and pH adjusted to 2 with phosphate acid) as mobile phase and detected by UV- detector at 230 nm. The results showed that Cu2 (ASP)4 and copper salicylate Cu2 (SA)4 could be separated satisfactorily. The detection limits of Cu2 (ASP)4 and Cu2 (SA)4 were 0.052 mg&middotL-1,0.04 mg&middotL-1 respectively and their corresponding average recoveries were 99.7 % ,99.9 % respectively.

关 键 词：高效液相色谱分析 阿斯匹林铜 水杨酸铜 药物测定 药理活性 质量标准 

分 类 号：R971[医药卫生—药品] R917[医药卫生—药学]