牛轮状病毒受体和人轮状病毒受体相似性的研究  

Similarity of Bovine rotavirus receptor and Human rotavirus receptor

作　　者：苏琦华[1] 訾自强[1] 潘菊芬[2] 徐燕[1] 

机构地区：[1]天津医科大学微生物学教研室,300070 [2]天津医科大学免疫学教研室,300070

出　　处：《中华微生物学和免疫学杂志》2002年第5期477-481,共5页Chinese Journal of Microbiology and Immunology

基　　金：天津市教委重点发展学科基金资助项目 (19940 4)

摘　　要：目的　应用抗牛轮状病毒受体单抗 ,探讨牛轮状病毒 (BRV)、人轮状病毒 (HRV)受体蛋白的相似性。为进一步研究受体性状、作用提供基础资料。方法　使用抗BRV R McAb进行ELISA、免疫斑点、细胞保护试验、固相分析及组织细胞的免疫组化染色。结果　发现抗BRV R McAb能和抗BRV IgG、抗HRV IgG结合 ,体外能特异性地阻断BRV、HRV攻击MA10 4细胞。提纯的BRV受体在体外能结合BRV、HRV。免疫组化试验证实 :在胎儿小肠粘膜、气管粘膜、MA10 4细胞膜表面有轮状病毒受体存在。结论　位于组织细胞膜表面的BRV、HRV受体能被 2种病毒识别 。Objective Monoclonal antibody against Bovine rotavirus (BRV) receptor (anti BRV R McAb) was used to find the similarity of BRV and Human rotavirus (HRV) receptor. Methods ELISA, dot immunobinding assay, cell protective assay, solid phase assay and immunohistochemistry method were applied. Results Anti BRV R McAb combined to both anti BRV IgG and anti HRV IgG and could protect MA104 cells against BRV and HRV attack. Immunohistochemistry tests showed that McAb combined with foetal intestinal, tracheal mucosa and MA104 cell membrane. We purified the rotavirus receptors on MA104 cells, which could combine both with BRV and HRV in vitro . Conclusion BRV receptor and HRV receptor are homogenous proteins and can be recognized by both BRV and HRV. [

关 键 词：单克隆抗体 轮状病毒受体 同源性蛋白 婴幼儿急性肠胃炎 免疫组织化学 

分 类 号：R373[医药卫生—病原生物学]