幽门螺杆菌中国菌株转位因子IS605的序列分析  

The sequence analysis of IS605 in Helicobacter pylori from China

作　　者：张茂俊[1] 张建中[1] 

机构地区：[1]中国预防医学科学院流行病学微生物学研究所腹泻室,北京102206

出　　处：《中华微生物学和免疫学杂志》2002年第5期493-495,共3页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金 (3 9870 0 3 2 );国家高技术研究发展计划 (863计划 )资助项目 (2 0 0 1AA2 15 1610 2 )

摘　　要：目的　研究幽门螺杆菌 (Hp)中国菌株转位因子IS6 0 5的序列差异。方法　采用PCR扩增、克隆、连接、测序的方法获得不同地区菌株IS6 0 5的序列并进行聚类分析比较。结果　中国菌株IS6 0 5的核苷酸序列高度保守 ,不同地区同源性达 93% ,相同地区同源性达 95 % ,G +C含量为 35 % ,与Hp的全基因相似。同源聚类分析表明相同地区IS6 0 5的序列相近。结论　IS6 0 5是幽门螺杆菌基因组中相当保守的成分 。Objective To analyze the conservation and diversity of IS605 of Helicobacter pylori in China. Methods PCR amplification and the PCR products were cloned into pGEM T vector and then sequenced respectively. The sequences were compared by Vector NTI Suite 6.0. Result The DNA sequence of IS605 is highly conserved. Their G+C contents were 35% similar with other genes in H.pylori . The DNA sequences of IS605 positive strains from the same region are highly homogeneous. Conclusion IS605 may be one ancient component of the Helicobacter pylori gene pool. We need advanced study to access the mechanisms of their transposition. [

关 键 词：中国菌株 序列分析 幽门螺杆菌 插入因子 IS605 

分 类 号：R378[医药卫生—病原生物学]