螺旋藻多糖抗HSV-1作用的体外实验研究  

Experimental Study on the Effects of Polysaccharides from Spirulina platensis (PSP) against Herpes Simplex Virus Type 1(HSV-1) in vitro

作　　者：于红[1] 张学成[1] 

机构地区：[1]青岛海洋大学海洋生命学院

出　　处：《高技术通讯》2002年第9期65-69,共5页Chinese High Technology Letters

基　　金：863计划 (819 0 4 10 )资助项目

摘　　要：报道从钝顶螺旋藻中提取的一种水溶性多糖类化合物 ,即钝顶螺旋藻多糖(PSP) ,在培养细胞内抗单纯疱疹病毒 1型 (HSV 1)作用的研究。以不同剂量的PSP作用于病毒复制周期的各个阶段 ,以病毒半数感染量 (TCID50 ) ,细胞病变 (CPE) ,蚀斑形成(PFU) ,MTT染色细胞保护率 (MTT法 )及核酸分子杂交作为评价指标 ,判断药效。结果表明 :PSP对Vero细胞毒性极低 ;对HSV 1无直接灭活作用 ,可干扰病毒向宿主细胞吸附 ,且经PSP预处理的细胞 ,能明显阻滞病毒产生细胞病变 ;PSP可有效地抑制病毒复制 ,但不影响病毒的释放 ;PSP可明显抑制HSV 1糖蛋白 gGmRNA的表达。提示PSP抗病毒靶位在于阻断病毒吸附和抑制感染细胞内病毒的复制及抑制HSV 1糖蛋白 gG基因的转录。In this paper we report the effect of polysaccharides from Spirulina platensis (PSP) against herpes simplex virus type 1(HSV 1) in Vero cells. PSP in various concentration was applied to different steps of HSV 1 replication cycle, the virus infectivity(TCID 50 ),cytopathic effect(CPE), plaque forming unit(PFU),MTT staining method for viable cells(MTT assay) and in situ hybrization were used as markers for monitoring virus growth. Results show that PSP had little cytotoxic effect on Vero cells,it could not directly inactivate HSV 1 infectivity,but interfer absorption of HSV 1 to Vero cells. PSP not only prevent HSV 1 infectivity when Vero cells were pretreated with PSP,but also inhibit HSV 1 replication in host cells. The polysaccharides inhibit the expression of HSV 1 gG mRNA,but do not show inhibition effect on HSV 1 release. These results suggest that some of the mechanisms of PSP against HSV 1 may be explained by the considerable inhibition effects on virus absorption,virus replication in cells and HSV 1 gG gene transcription.

关 键 词：体外实验研究 螺旋藻多糖 单纯疱疹病毒1型 抗病毒作用机制 HSV-1 抗病毒药物 

分 类 号：R285.5[医药卫生—中药学]