利用目的基因转化技术培育棉花抗病新品种  

Breeding Fusarium Wilt and Verticillium Wilt of Cotton-Resistant Strains With Transgene Technique

作　　者：乐锦华[1] 祝建波[1] 崔百明[1] 朱新霞[1] 王爱英[1] 陈正华[2] 刘桂珍[2] 李国英[3] 简桂良[4] 吴刚 

机构地区：[1]石河子大学生物工程学院,新疆石河子832003 [2]中国科学院遗传研究所,北京100101 [3]石河子大学农学院农业科学系,新疆石河子832003 [4]中国农科院植物保护研究所,北京100094 [5]农一师农科所,新疆阿拉尔843300

出　　处：《石河子大学学报（自然科学版）》2002年第3期173-178,共6页Journal of Shihezi University(Natural Science)

基　　金：国家计委"八五"重点科技攻关项目 (85 72 2 2 4 0 2 )

摘　　要：用花粉管通道法将菜豆几丁质酶基因与烟草 β 1、3葡聚糖酶基因双价植物表达载体pBLGC导入新疆棉花主栽品种 (系 )系 5 5 0、82 2、130 4、石远 32 1。通过卡那霉素检测、PCR Southern验证、抗黄萎病、枯萎病性筛选 ,得到抗病性良好且遗传性稳定的转基因棉花。经过 6年 10个世代的选育 ,育成抗枯萎病、抗 (耐 )Using pollen tube pathway methed the bivalent plant propess carrier for bean chitin enzyme gene & tobacco β 1,3 glucanose gene were transferred to the strains of cotton in local,550,1304,822 and shiyuan 321.Pass through kanamycin check, PCR Southern blot test, screen for the character of fusarium wilt and verticillum wilt of cotton resistant, the transgenic plant of cotton that had good resistant and stable heredity were obtained.After selection for 10 generations the new strains of cotton of fusarium wilt and verticillum wilt of cotton resistant or endurance were bred.

关 键 词：目的基因转化技术 棉花 枯萎病 黄萎病 转基因棉花 花粉管通道法 抗病品种 良种培育 

分 类 号：S562.034[农业科学—作物学] Q943.2[生物学—植物学]