一株茶碱降解菌的分离和鉴定  

Isolation and Identification of a Theophylline-degrading Bacterial Strain

作　　者：许玫英[1] 岑英华[2] 邓穗儿[2] 孙国平[2] 

机构地区：[1]中国科学院武汉病毒研究所,武汉430071 [2]广东省微生物研究所

出　　处：《微生物学报》2002年第5期526-533,共8页Acta Microbiologica Sinica

基　　金：广东省自然科学基金资助项目

摘　　要：从制药废水生物处理系统的活性污泥中经富集分离到一株能以茶碱作为唯一碳、氮源生长的茶碱降解菌Tcn3 ,该菌株可以利用茶碱的最高浓度为 3 0 0 0mg L。当茶碱浓度为1 0 0 0mg L时被彻底降解的时间仅需 48h。Tcn3菌株降解茶碱的最适pH为 8 0。K+ 是该菌株降解茶碱的必需元素。采用 1 6SrDNA序列分析法及传统的生理生化特征鉴定法对该菌株进行鉴定 ,结果表明 ,Tcn3的 1 6SrDNA的核苷酸序列与善变副球菌 (Paracoccusversutus)ATCC2 5 3 6 4的同源性为 99 7% ,在细菌系统发育分类学上属于变形菌α亚类 ,Rhodobacter组 :副球菌属 。Bacterial strain Tcn3 was isolated from the activated sludge of a pharmaceutical wastewater treatment system, which could grow with theophylline as the sole source of carbon and energy. The maximum concentration of theophylline degraded by Tcn3 is 3 000mg/L. When the concentration of theoplyline is 1 000mg/L, a complete degradation only needs 48h. For Tcn3 degrading theophylline, the optimal pH is 8^0 and K++ is the necessary element. The sequence analysis of a 1 500bp 16SrDNA fragment amplified from total DNA of strain Tcn3 and the traditional identification methods was used to identify Tcn3. The results showed that Tcn3 shares 99.7% 16S rDNA sequence homology with Paracoccus versutus type strain ATCC 25364. In phylogenetic framework of bacterial classification, Tcn3 belongs to proteobacteria; alpha subdivision; Rhodobacter group: Paracoccus versutus.

关 键 词：茶碱降解菌 分离 鉴定 16SrDNA 善变副球菌 制药厂废水 

分 类 号：Q939.9[生物学—微生物学]