共表达MDV糖蛋白B和鸡γ干扰素基因的重组鸡痘病毒的构建  

Construction of Recombinant Fowlpox Virus Coexpressing Marek's Disease Virus gB and Chicken Type Ⅱ Interferon Genes

作　　者：彭大新[1] 刘秀梵[1] 程坚[1] 周春红[1] 陈素娟[1] 

机构地区：[1]扬州大学畜牧兽医学院,扬州225009

出　　处：《微生物学报》2002年第5期611-615,共5页Acta Microbiologica Sinica

基　　金：国家 8 6 3计划 ( 96 0 332 0 2 5 );上海市科技兴农重点攻关项目 (农科攻字 ( 2 0 0 0 )第 5 3号 );江苏省自然科学基金项目 (BK2 0 0 1 4 1 5 )~~

摘　　要：Recombinant fowlpox viruses(rFPVs)coexpressing MDV gB and chicken type Ⅱ interferon (IFN-Ⅱ) gene were constructed by using different promoters of Ps and PE/L.rFPVs were selected and purified by blue plaque expressing β-galactosidase.The expression of MDV gB gene was confirmed by indirect immunofluorescence assay.The levels of MDV gB expressed in rFPV-gB-IFN under the control of the synthetic promoter Ps was same as that in rFPV-gB.The expression of IFN-Ⅱ was examined in chicken embryo fibroblast(CEF) by protection against vesicular stomatitis virus-induced cytopathic effect.The levels of IFN expressed in CEF was 640～1280 units/mL.When rFPVs were propagated in CEF,the plaque sizes of rFPV-gB were larger than those of rFPV-gB-IFN,the plaque formation units of rFPV-gB were also more than those of rFPV-gB-IFN.Recombinant fowlpox viruses(rFPVs)coexpressing MDV gB and chicken type Ⅱ interferon (IFN-Ⅱ) gene were constructed by using different promoters of Ps and PE/L.rFPVs were selected and purified by blue plaque expressing β-galactosidase.The expression of MDV gB gene was confirmed by indirect immunofluorescence assay.The levels of MDV gB expressed in rFPV-gB-IFN under the control of the synthetic promoter Ps was same as that in rFPV-gB.The expression of IFN-Ⅱ was examined in chicken embryo fibroblast(CEF) by protection against vesicular stomatitis virus-induced cytopathic effect.The levels of IFN expressed in CEF was 640～1280 units/mL.When rFPVs were propagated in CEF,the plaque sizes of rFPV-gB were larger than those of rFPV-gB-IFN,the plaque formation units of rFPV-gB were also more than those of rFPV-gB-IFN.

关 键 词：共表达 MDV糖蛋白B 鸡γ干扰素基因 重组 鸡痘病毒 

分 类 号：Q782[生物学—分子生物学]