环状芽孢杆菌几丁质酶基因序列分析、表达和生物活性测定  

Study on the Sequence Analysis,Expression and Application ofChitinase Gene From Bacillus circulans

作　　者：王焰玲[1] 王海燕[1] 秦敏[1] 张义正[1] 

机构地区：[1]四川大学生命科学学院四川省分子生物学与生物技术重点实验室,成都610064

出　　处：《微生物学报》2002年第5期616-619,共4页Acta Microbiologica Sinica

基　　金：国家自然科学基金资助项目 ( 39770 4 1 2和 39876 4 0 0 )~~

摘　　要：Sequence analysis of the chitinase gene cht1 of Bacillus circulans showed that it contained 2151 nucleotides,which codes the precursor of chitinase CHT1 with 717 amino acid residues.The nucleotide and deduced amino acid sequences of cht1 showed 81% and 95% homology with those of ChiA of B.circulans WL-12,respectively.The cht1 gene was cloned into the Escherichia coli-Bacillus subtilis shuttle vector pSUGV4 and two recombinant plasmids, named pUSCH1 and pUSCH2 which contained 2.9kb and 4.0 kb insert respectively,were obtained.The recombinant plasmids were transformed into B.subtilis DB104 and WB600.Chitinase activity was detected both in transformed E.coli and B.subtilis.The DB104/pUSCH1 strain was found to be effective in the bio-controlling the infection of Magnaporthe grisea under greenhouse condition,which showed 71.67% decrease in rice disease incidence.Sequence analysis of the chitinase gene cht1 of Bacillus circulans showed that it contained 2151 nucleotides,which codes the precursor of chitinase CHT1 with 717 amino acid residues.The nucleotide and deduced amino acid sequences of cht1 showed 81% and 95% homology with those of ChiA of B.circulans WL-12,respectively.The cht1 gene was cloned into the Escherichia coli-Bacillus subtilis shuttle vector pSUGV4 and two recombinant plasmids, named pUSCH1 and pUSCH2 which contained 2.9kb and 4.0 kb insert respectively,were obtained.The recombinant plasmids were transformed into B.subtilis DB104 and WB600.Chitinase activity was detected both in transformed E.coli and B.subtilis.The DB104/pUSCH1 strain was found to be effective in the bio-controlling the infection of Magnaporthe grisea under greenhouse condition,which showed 71.67% decrease in rice disease incidence.

关 键 词：环状芽孢杆菌 几丁质酶 基因序列分析 表达 生物活性 测定 枯草芽孢杆菌 稻瘟病菌 真菌病害 

分 类 号：Q936[生物学—微生物学] S432.44[农业科学—植物病理学]