蛙病毒核糖核酸酶Ⅲ基因结构及其序列分析  

Identification and Characterization of the RNase Ⅲ Gene of the Tiger Frog Virus

作　　者：吕玲[1] 何建国[1] 何华虹[1] 邓敏[1] 翁少萍[1] 王晓红[1] 

机构地区：[1]中山大学生命科学学院,广东广州510275

出　　处：《中国病毒学》2002年第3期257-262,共6页Virologica Sinica

基　　金：国家"973"项目 (G19990 12 0 10 ) ;广东省自然科学基金资助项目 (990 2 5 5 )

摘　　要：对蛙病毒 (TFV)核糖核酸酶Ⅲ基因序列进行分析。TFV基因组中含有完整的核糖核酸酶Ⅲ基因序列 ,全长为 1113bp ,GC含量为 5 6 .6 3%。其推定蛋白质的分子量为 4 0 .4 7kD ,等电点为7.99。序列结构分析发现在编码区的下游有可形成茎环的反向重复序列和形成发夹结构的回文序列。与其它物种相比 ,TFV与虹彩病毒的LCDV 1和CIV的核糖核酸酶Ⅲ基因的氨基酸序列同源性较高 ,与酵母。From the tiger frog virus(TFV) genome, an open reading frame(ORF), whose deduced amino acid sequence (aa) shows identity with RNase Ⅲ,was identified. The ORF has 1 113bp long and codes a putative protein of 371 aa with a predicted molecular mass of 40.47kD and a G+C content of \{56.63%\}. Stem loops and short dyad sequences appear in the downstream of the translation stop codon. Compared with the RNase Ⅲs found in TFV and other species, the higher homology among TFV and the two iridovirus is present, and TFV has lower identity with other species, especially yeast and rhabditida.

关 键 词：蛙病毒 核糖核酸酶Ⅲ 基因结构 序列分析 

分 类 号：S917.1[农业科学—水产科学] S947.2