葡萄球菌B型肠毒素突变体的分子设计、高效表达与活性初步研究  

Study on the molecular design,high-level expression and activity of mu tant staphylococcal enterotoxin B

作　　者：姜永强[1] 郑玉玲[1] 宁保安[2] 马茹[1] 李韩平[3] 王景林[1] 

机构地区：[1]军事医学科学院微生物流行病研究所,北京100071 [2]军事医学科学院卫生和环境医学研究所,天津300050 [3]陕西师范大学运动生物学研究所,西安710062

出　　处：《生物技术通讯》2002年第5期341-343,共3页Letters in Biotechnology

摘　　要：葡萄球菌B型肠毒素(SEB)是重要的超抗原,依据SEB分子的晶体结构并结合表面分析,模拟设计了与MHCⅡ类分子和TCRVβ区亲合力降低的三位点突变体mSEB(Y89A,C93S,Y94A)。通过PCR重叠延伸法获得突变体基因,导入PBV220载体中,于E.coliDH5α中获得高效表达,纯化的突变毒素T细胞激活活性仅为野生型毒素的1/5左右。Mutant staphylococcal enterotoxin B,which was suggested to have a low affinity to both MHC-II complex and T Cell receptor,was designed by3-D structure and surface property analysis.The mutant gene was constructed by PCR over-extension method and inserted to prokaryotic expression vector PBV220for a high expression in E.coli system.Moreover,the purified mutated products showed only1/5T cell stimulation potentency of the wild-type Staphylococcal enterotoxin B.

关 键 词：葡萄球菌B型肠毒素突变体 分子设计 高效表达 活性 定点突变 超抗原 

分 类 号：R378.11[医药卫生—病原生物学] R996.2[医药卫生—基础医学]