猪1号染色体微卫星多态性研究及遗传连锁图谱的构建  

The Microsatellite Polymorphism Research on Porcine Chromosome 1 and the Construction of Its Genetic Map

作　　者：屈彦纯[1] 邓昌彦[1] 熊远著[1] 苏玉虹[1] 郑嵘[1] 刘桂兰[1] 

机构地区：[1]华中农业大学畜牧兽医学院农业部猪遗传育种重点实验室,武汉430070

出　　处：《遗传》2002年第5期539-542,共4页Hereditas（Beijing)

基　　金："973"项目G2000016105;国家高技术研究发展计划专项经费资助

摘　　要：微卫星具有多态性高、保守性好等优点。本研究选取以20cM左右的间距均匀分布于1号染色体的8个多态微卫星基因座构建猪1号染色体的遗传连锁图谱,为进一步进行重要经济性状基因座的定位打下基础。试验结果表明,8个基因座等位基因数目2～5个,各个基因座等位基因频率在0.015～0.75之间,杂合度为0.39705～0.67675,多态信息含量为0.32925～0.59316。构建的资源家系遗传连锁图谱总长181.5cM,与USDA结果基本一致,可进一步用于猪数量性状基因座定位的研究。As a molecular marker,microsatellite has many advantages such as high polymorphism and good conserva-tiveness in animal genetic research. The study chose 8 microsatellite markers that evenly distributed on chromosome 1 with a distance about 20 cM to build the genetic map of porcine chromosome 1. The results of our experiment are as follows:the number of alleles for 8 markers is 2 to 5,their gene frequency is from 0. 015 to 0. 75,the heterozygosi-ty is from 0. 39705 to 0. 67675 and the polymorphic information content is from 0. 32925 to 0. 59316. The map we built is basically in consistent with the result of USDA and can be used in searching quantitative traits loci in pigs.

关 键 词：猪 1号染色体 微卫星多态性 遗传连锁图谱 

分 类 号：Q953[生物学—动物学]