In Vitro Fertilization in Maize and Tobacco: The Fusion Patterns During Sexual Cell Fusion  

体外受精研究 :玉米和烟草性细胞融合的几种模式(英文)

作　　者：孙蒙祥[1] 杨弘远[1] Mauro CRESTI 

机构地区：[1]武汉大学植物发育生物学教育部重点实验室,武汉430072 [2]Depart ment of Environmental Science

出　　处：《Acta Botanica Sinica》2002年第10期1142-1145,共4页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家基础研究发展规划项目 (G1 9990 5 390 9);国家自然科学基金 ( 39970 368);欧盟合作项目 ( 1 8CT960 0 72 )。~~

摘　　要：Large scale of sexual cell fusion was carried out in order to observe the fusion pattern during in vitro fertilization with special interest in the influence of cell volume on the membrane behavior. Three patterns could be recognized in sexual cell fusion, which was supported by fluorescent microscopy with the aid of video enhanced system and cooled CCD. It was found that the fusion pattern and cell membrane behavior were related to the cell volume ratio of two fusing cells. The results reported here might be useful for interpreting and evaluating data from in vitro fertilization experiments and for explaining male cytoplasm exclusion during fertilization.分别用玉米 (ZeamaysL .)和烟草 (NicotianatabacumL .)为材料 ,以单对细胞融合技术广泛开展了各种组合的性细胞的融合实验。重点观察了性细胞融合过程中细胞体积对细胞膜融合行为的影响。视频增差显微术结合细胞膜荧光标记技术显示 ,性细胞融合过程中受融合双方体积差异大小的调节 ,细胞膜的融合行为可归纳为 3种基本模式 ;发现在体外受精条件下精细胞可以类似被吞噬的方式进入卵细胞 ,且其融合动作非常迅速。由此提出一种假设机制用以解释体内受精过程中雄性细胞质被排除的可能原因。

关 键 词：in vitro fertilization MAIZE TOBACCO cell volume fusion pattern FITC-Con A 

分 类 号：Q813.2[生物学—生物工程]