Cell Growth and Ajmalicine Accumulation in a Full Habituated Catharanthus roseus Cell Line C_ (20)hi  

长春花激素完全适应型细胞的生长和阿玛碱合成特性(英文)

作　　者：郑珍贵[1] 周煜[1] 刘涤[1] 胡之璧[1] 

机构地区：[1]上海中医药大学中药研究所,上海200032

出　　处：《Acta Botanica Sinica》2002年第10期1146-1150,共5页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家中医药管理局青年基金 ( 97Y0 1 0 );上海市教育委员会科技发展基金 ( 940 0 9)。~~

摘　　要：A full habituated cell line C_ 20hi was screened from 2,4_D dependent line (C_ 20D) of Catharanthus roseus (L.) G. Don. The investigation involved the cell growth, ajmalicine production and enzyme activity related to indole alkaloid biosynthesis in both cell lines. These results indicated that C_ 20hi cells grew faster than C_ 20D cells, and average ajmalicine content in C_ 20hi cells was 18.4 times more than that in C_ 20D when cultured in the production medium. In the growth medium, average ajmalicine content in C_ 20hi cells was 31.9 times more than that in C_ 20D cells, while the cell growth has no obvious difference. The comparison of enzyme activities in C_ 20hi and C_ 20D cells indicated that tryptophan decarboxylase (TDC), strictosidine synthase (SSS) and geraniol_10_dehydrogenase (G10H) activities have no close relation to ajmalicine accumulation, although the activities of these enzymes were higher when cells were cultured in the production medium than in the growth medium. The C_ 20hi cells are relatively stable in five years of culture.从长春花激素依赖型细胞系 (C2 0 D)筛选出一种激素完全适应型的细胞系 (C2 0 hi) ,考察了两种细胞生长、阿玛碱合成和与吲哚生物碱生物合成相关的酶的活性 ,结果表明 :在生长培养基上二者生长无显著差异 ,而C2 0 hi细胞平均阿玛碱含量是C2 0 D的 31 9倍 ,在生产培养基上C2 0 hi细胞生长较C2 0 D快 ,C2 0 hi平均阿玛碱含量是C2 0 D的1 8 4倍。通过比较生产和生长培养基中C2 0 hi细胞的色氨酸脱羧酶、异胡豆苷合酶和牛儿醇_1 0_脱氢酶活性 ,发现在生产培养基中培养细胞的 3种酶活性均显著高于生长培养基 ,但与阿玛碱积累没有密切关系。研究结果还表明 ,通过 5年的继代培养 ,激素完全适应型细胞系C2 0 hi的阿玛碱含量是比较稳定的。

关 键 词：full habituated cells AJMALICINE cell culture Catharanthus roseus 

分 类 号：Q945[生物学—植物学]