Mechanism of Oxidative Burst in Tobacco Leaves and Cells Induced by Palmin from Phytophthora palmi  

Pal min诱导烟草氧化猝发的机制(英文)

作　　者：王义华[1] 张宏明[1] 于中连[1] 陈珈[1] 王学臣[1] 

机构地区：[1]中国农业大学生物学院植物生理生化国家重点实验室,北京100094

出　　处：《Acta Botanica Sinica》2002年第10期1160-1166,共7页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家重点基础研究发展规划项目 (G1 9990 1 1 70 0 );国家自然科学基金 ( 30 1 70 0 889)。~~

摘　　要：In order to reveal the signaling pathways triggered by elicitor in plant-microbe interactions, the mechanisms of hypersensitive necrosis responses in Nicotiana tabacum L. cv. Gexin III induced by palmin were studied at molecular and cellular level. The burst of superoxide, intercellular diffusion of hydrogen peroxide and process of cell death induced by palmin were investigated in tobacco plants by biochemical methods and Confocal microscopy. The results showed that a large amount of O-2(.-) was rapidly generated in tobacco cell elicited by palmin as a result of activation of NADPH oxidase, and the O-2(.-) was dismutated into H2O2 immediately by superoxide dismutase (SOD). Accumulation and intercellular diffusion of H2O2 were shown to be a trigger for hypersensitive cell death; and Ca2+ and some specific protein kinase were also shown to be involved in the activation of oxidative burst in tobacco cell induced by palmin.为探讨植物对病原微生物的防御机制和激发子启动植物体内的信号转导应答过程 ,本文研究了Phytoph thorapalmi激发子palmin诱导其非寄主亲和性烟草的叶片和悬浮细胞系产生氧化猝发的分子机理。利用生化分析和激光共聚焦显微扫描技术动态观察palmin诱导烟草过敏反应中O·- 2 和H2 O2 的形成、胞间转移及引起细胞死亡的特性。结果表明 :palmin诱导激活了烟草细胞内NADPH氧化酶 ,产生大量的O·- 2 ;O·- 2 在SOD催化下迅速转变成H2 O2 ,并且H2 O2 在一定范围的细胞间转移和积累 ,最后诱发烟草细胞的过敏性坏死反应。palmin诱导氧化猝发过程还有Ca2 +和蛋白激酶的参与。

关 键 词：oxidative burst NADPH oxidase SOD ELICITOR Nicotiana tobacum 

分 类 号：S432[农业科学—植物病理学]