DIG标记DNA探针检测noggin在爪蟾胚胎中的表达  

Detection of Expression Distribution of Noggin in Xenopus Embryos with DIG-labeled DNA Probe

作　　者：周文[1] 冯湘玲[1] 杨旭宇[1] 王磊[1] 姚开泰[1] 

机构地区：[1]中南大学湘雅医学院肿瘤研究所,中国湖南长沙410078

出　　处：《生命科学研究》2002年第3期239-242,共4页Life Science Research

基　　金：国家自然科学基金资助项目 ( 39910 16 1994)

摘　　要：整体原位杂交 (Whole mountinsituhybridization)用于基因表达定位和表达分布模式的研究 ,已经成为一种非常重要的手段 .PCR方法进行探针标记 ,可以获得特异性高 ,片断大小可变的探针 .采用DIG标记 ,检测已知基因noggin在爪蟾胚胎时空分布 ,所得结果与文献报道一致 。Whole-mount in situ hybridization has become a very important tool for studying localization and distribution pattern of gene expression.Highly specific probes were obtained,the length of which are variable.With DIG labeling,the temporal and spatial expression of noggin,one of known genes,was detected.The results obtained were consistent with those in paper previously reported,suggesting that the DNA probe produced by PCR is suitable for whole-mount in situ hybridization on Xenopus embryos under specific conditions.

关 键 词：DIG标记 DNA探针 检测 NOGGIN 爪蟾胚胎 表达 整体原位杂交 

分 类 号：Q344.5[生物学—遗传学] Q786