锂对铅引起的大鼠海马CA1区长时程增强效应(LTP)损伤的修复作用  

LITHIUM RESTORES THE LEAD-INDUCED IMPAIRMENT OF THE LONG-T ERM POTENTIATION IN THE CA1REGION OF RAT HIPPOCAMPUS

作　　者：钟桂生[1] 姚颖[1] 阮迪云[1] 

机构地区：[1]中国科学技术大学生命科学学院,安徽合肥230027

出　　处：《生物物理学报》2002年第3期293-296,共4页Acta Biophysica Sinica

基　　金：中国科学院创新项目(KZCX2-410);国家自然科学基金项目(30170809;30000039);教育部博士点基金项目(1999035811);中国科大基金项目(KY1504;KY1206)

摘　　要：应用离体脑片记录技术,记录大鼠海马CA1区的兴奋性突触后电位(EPSPs),研究了锂对铅引起的大鼠海马CA1区长时程增强效应(long-termpotentiation,LTP)损伤的修复作用。结果表明:对照组大鼠海马CA1区LTP幅度为194.42±14.05%(n=10);铅处理组LTP的幅度为147.06±9.55%(n=13);而锂加铅处理组LTP的幅度为193.45±14.91%(n=15)。与对照组相比,铅处理组LTP的幅度降低了47.36%,而锂几乎完全修复了铅对大鼠海马CA1区LTP幅度的损伤。锂和铅处理后对大鼠海马CA1区的双脉冲易化(paired-pulsefacilica-tion,PPF)都有一定的抑制作用,在脉冲间隔为50ms时,这种抑制效应最大:对照组为155.58±6.35%(n=7);铅暴露组为150.26±13.74%(n=8);锂加铅处理组为140.59±15.42%(n=8)。结果表明:锂对铅引起大鼠海马CA1区LTP的损伤有一定的修复作用。The protective effects o f lithium on the lead-induced impairment of long-term potentiation (LTP) were studied by recording excitatory postsynaptic potentials (EPSPs) in CA1 region of rat hip-pocampus in vitro. The LTP amplitude in hippoca mpus slices of control rats, chronic lead exposure rats and chronic l ithium plus lead administration rats are 194.42±14.05%(n=10); 147.06±9.55 %(n=13) and 193.45±14.91%(n=15) respectively. Chronic lead exposure inhib ited LTP amplitude while chronic lithium administration almost restores the impairment of LTP by lead exposure. Chronic lead and lithium exp o-sure differently inhibited paired-pulse facilitation (PPF) and the inhi bitory effect was largest when PPF was measured at 50ms inter-pulse in terval. The PPF value in control: 155.58±6.35%(n=7);Pb: 150.26±13.74% (n=8);PbLi: 140.59±15.42%(n=8). The results showed that chronic lith ium adminis-tration may protect the impairment of LTP caused by chroni c lead exposure in CA1 region of rat hip-pocampus.

关 键 词：大鼠 海马 CA1区 修复作用 锂 铅 脑片 长时程增强效应 LTP 神经毒性 神经损伤 

分 类 号：R992[医药卫生—毒理学] R994.6[医药卫生—药学]