根癌农杆菌转化紫草的研究  

Study on the Genetic Transformation of Lithospermum erythrorhizon by Agrobacterium tumefaciens

作　　者：祁建军[1] 宋经元[1] 雷和田[1] 张荫磷[1] 

机构地区：[1]中国医学科学院药用植物研究所中国协和医科大学,北京100094

出　　处：《云南植物研究》2002年第5期656-658,共3页Acta Botanica Yunnanica

摘　　要：Crown gall tissue culture lines of Lithospermum erythrorhizon were established by transformation of sterile seedlings with Agrobacterium tumefaciens C58. Nopaline was inspected in the crown gall and showed that the transformation was successfull. L6 line, can secret red naphthoquinone pigments that are shikonin derivatives on phytohormone free 6,7-V medium, was selected from other lines on different basic phytohormone free media(MS, 6,7-V, WP, B5).The content of shikonin in the crown gall was 1 18%(dry tissue).The study showed that crown gall tissue culture of L.erythrorhizon was a new method to produce shikonin.Crown gall tissue culture lines of Lithospermum erythrorhizon were established by transformation of sterile seedlings with Agrobacterium tumefaciens C58. Nopaline was inspected in the crown gall and showed that the transformation was successfull. L6 line, can secret red naphthoquinone pigments that are shikonin derivatives on phytohormone free 6,7-V medium, was selected from other lines on different basic phytohormone free media(MS, 6,7-V, WP, B5).The content of shikonin in the crown gall was 1 18%(dry tissue).The study showed that crown gall tissue culture of L.erythrorhizon was a new method to produce shikonin.

关 键 词：根癌农杆菌 转化 紫草 冠瘿组织培养 红色株系 

分 类 号：Q943.1[生物学—植物学]