大、小动脉内皮细胞乙酰胆碱作用靶标药理学特性的比较  

COMPARISON OF PHARMACOLOGICAL CHARACTERISTICS OF THE ENDOTHELIAL TARGET FOR ACETYLCHOLINE BETWEEN BIG ARTERY AND SMALL ARTERY

作　　者：贾国栋[1] 龙超良[1] 刘国树[2] 汪海[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850 [2]解放军总医院南楼心肾科,北京100853

出　　处：《中国应用生理学杂志》2002年第3期252-256,共5页Chinese Journal of Applied Physiology

基　　金：国家重点基础研究发展规划项目(国家 973计划 :G19980 5 112 );赛德维康医药研究院科技基金项目

摘　　要：目的 :比较大、小动脉内皮细胞乙酰胆碱作用靶标药理学特性的差异。方法 :采用大鼠尾动脉螺旋状血管条和主动脉离体血管环两种组织 ,对比观察乙酰胆碱 (ACh)诱发大、小动脉内皮依赖性舒张反应特征的差异 ,从而进一步研究小动脉内皮细胞乙酰胆碱作用靶标的药理学特性。结果 :氯化钾 (6 0mmol/L)预致血管收缩的尾动脉和主动脉对不同浓度ACh (10 -8～ 10 -4mol/L)产生内皮依赖性舒张反应 ,且呈剂量依赖性。L Nω 硝基精氨酸甲酯 (L NAME :10 -4mol/L)或美蓝 (MB :10 -5mol/L)与吲哚美辛 (Indo :10 -4mol/L)联用仅可部分地阻断ACh诱发尾动脉内皮依赖性舒张反应 ;而L NAME或MB可完全阻断ACh诱发主动脉内皮依赖性舒张反应。结论 :ACh激活大、小动脉上内皮细胞乙酰胆碱作用靶标诱发内皮依赖性舒张反应的药理学性质不同 ,在小动脉上 ,除了NO和PGI2介导外 ,还有一种非NO和非PGI2 的舒血管因子参与 ;在大动脉上 。Aim: To compare the differences of pharmacological characteristics of the endothelial target for acetylcholine(ETA) between rat aorta and tail artery. Methods: Differences in the endothelium dependent relaxation induced by acetylcholine (ACh: 10 -8 ～10 -4 mol/L) were studied using isolated rat tail artery helical strips and aortic rings, so that the pharmacological characteristics of ETA in small artery can be observed. Results: ACh induced endothelium dependent relaxation was observed both in rat tail artery strips and in aortic rings precontracted with potassium chloride (60 mmol/L) in a concentration dependent manner. In tail artery this effect was partially blocked by L N ω Nitro arginine methyl ester (L NAME: 10 -4 mol/L) or methylene blue (MB: 10 -5 mol/L), together with indomethacin (Indo: 10 -4 mol/L), but in aorta it was completely blocked by L NAME or MB. Conclusion: It is different of the pharmacological characteristics of ETA between big artery and small artery. A non NO and non PGI 2 relaxing factor, together with nitric oxide (NO) and prostacyclin (PGI 2), mediates endothelium dependent vasorelaxation induced by ACh in small artery, but NO may be the principal endothelial vasodilator substance in big artery.

关 键 词：小动脉 内皮细胞 乙酰胆碱作用靶标 药理学特性 比较 尾动脉 主动脉 内皮依赖性舒张 大动脉 

分 类 号：R963[医药卫生—微生物与生化药学]