小鼠粗线期精母细胞钙离子通道膜片箝方法的建立  

Study on Ca^(2+) Channels of Mouse Pachytene Spermatocytes Using Patch Clamp

作　　者：张莉[1] 张习春[1] 高蓉[1] 肖杭[1] 

机构地区：[1]南京医科大学毒理学研究所神经毒理室,南京210029

出　　处：《上海实验动物科学》2002年第3期138-140,144,共4页Shanghai Laboratory Animal Science

基　　金：国家自然科学基金资助项目 (30 170 811)

摘　　要：应用机械分离获得小鼠单个精母细胞 ,采用全细胞膜片箝技术记录 Ca2 +离子通道电流。结果表明 :1阻断 K+电流后 ,当钳制电位 - 90 m V、指令电压 - 6 0～ + 10 m V、步阶电压 10 m V时 ,可记录到内向电流 ;2内向电流在 - 30～ - 40 m V达到最大值 ;3在细胞外液中加入 4 μmol/ L TTX,对记录电流无影响 ,表明此电流不含有 Na+电流成分 ,为 Ca2 +电流。Ca 2+ currents were obtained in acutely dissociated mouse spermatogenic cells using whole cell patch clamp technique. The results indicated that inward currents were recorded when K + currents were blocked by Cs + and TEA + stepping membrane potential to voltage between -80 and +10 mV, in 10 mV increments from a holding potential of -90 mV, the peak amplitude occurred at between -30 mV and -40 mV,and the inward currents remained unchanged in the presence of 4 μmol/L tetrodoxin(TTX), a special sodium channels blocker, suggesting that it was generated by opening Ca 2+ channels other than Na + channels.

关 键 词：小鼠 粗线期 精母细胞 全细胞膜片箝技术 钙离子通道 

分 类 号：Q954.53[生物学—动物学]