海水淹溺大鼠肺脏内皮素及降钙素基因相关肽含量的变化  

Changes of ET and CGRP content in the lungs of the rats drowned in seawater

作　　者：姜忠华[1] 范静[2] 强琳[1] 辛苏宁 滕燕生 楚立云[1] 贾钢锐 钟广文[1] 吴力克 

机构地区：[1]解放军第四○一医院神经内科,青岛266071 [2]青岛大学医学院急诊科 [3]解放军第四○一医院干部病房科,青岛266071 [4]解放军第四○一医院航海病中心,青岛266071 [5]解放军第四○一医院中心实验室,青岛266071

出　　处：《中华航海医学与高气压医学杂志》2002年第3期152-154,共3页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

摘　　要：目的　研究低温海水淹溺大鼠肺脏内皮素 (ET)及降钙素基因相关肽 (CGRP)含量变化规律 ,探讨二者对肺脏功能的影响。方法　将大鼠随机分为对照组和淹溺组。淹溺组大鼠分别置于 16～ 18℃海水中自由游动 ,于淹溺后 5 ,15 ,30 ,6 0 ,2 4 0 ,36 0 m in及死亡后测量肛温 ,自左心室取血 1ml行血气分析 ,处死动物取左肺计算肺干湿重比值 ,取右肺检测 ET和 CGRP含量。结果　海水淹溺后 ,不同时间组动物肛温、肺组织干湿重比值、p H、Pa O2 明显降低 ,其中肛温、p H、Pa O2 于 5 min时降至最低点 ,肺组织干湿重比值于 6 0 m in降至最低点 ;Pa CO2 则明显升高 ,于 5 m in组升至最高点 ;随后上述指标呈恢复趋势 ,然而除 p H于 2 4 0及 36 0 min时接近正常对照水平外 (P>0 .0 5 ) ,其余均仍未恢复到正常对照组水平 (P<0 .0 1)。 ET含量于 30 min和 CGRP含量于 15 m in分别降至最低点 [(15 .6 8± 6 .5 3) ng/ L及(34.4 7± 5 .5 8) ng/ L ],与正常对照组相比差异有显著性 (P<0 .0 1) ,随后呈上升趋势 ,前者至 2 4 0 m in高达 (5 8.92± 6 .39) ng/ L ,比正常对照组 (30 .72± 7.0 6 ) ng/ L明显增高 (P<0 .0 1) ,36 0 min时 (2 5 .6 9±4 .2 7) ng/ L恢复接近正常对照组水平 (P>0 .0 5 ) ;后者至 36 0 m in时 (49.Objective To study changes of ET and CGRP in the lungs of the rats drowned in hypothermic seawater and to analyse its influence on the function of the lungs. Methods Rats in different groups were put into 16~18 ℃ hypothermic seawater alternately and let swim freely. 5,15,30,60,240,360 min after drowning and in death, the rectal temperature of the rats was examined respectively, also blood gas was analyzed with 1 ml arterial blood taken from left ventricle rapidly, and the ratio of dry lung to wet lung was counted after death, and the ET and CGRP content in lungs were examined. Results The rectal temperature, the ratio of dry lung to wet lung, and pH,PaO 2 went down; rectal temperature, pH and PaO 2 reached the lowest point in 5 minutes; ratio of dry lung to wet lung reached the lowest point in 60 minutes. Afterwards all the above indexes showed a rising tendency. However except that pH went near the level of the normal control group in 240 and 360 minutes( P >0 05), the other indexes still did not reached the level of the normal control group( P <0 01). ET and CGRP content in lungs went down to the lowest point in 30 min and 15 min respectively after drowning in seawater, and then showed a rising tendency; ET became higher than the level of the control group in 240 minutes and, went near the level of the control group in 360 minutes; while CGRP did not go near the level of the control group in 360 minutes. There was no correlation between ET and CGRP ( r =0.038, P >0 05). Conclusions The production and secretion of ET and CGRP were influenced by hypothermia,anoxemia and acidosis. They may play an important role in the process of lung injury, and there was no linear correlation between ET and CGRP.

关 键 词：低温海水 淹溺 内皮素 降钙素基因相关肽 

分 类 号：R83[医药卫生—航空、航天与航海医学]