原发性高血压和妊娠高血压患者血清中钠泵抑制因子免疫活性物的测定  

Measurement of Endogenous Sodium Pump Inhibitor in Serum of Patients with Essential Hypertension and Pregnancy Hypertension

作　　者：李素琴[1] 符云峰[1] 王欣华 朱树森[1] 

机构地区：[1]河北省医学科学院实验医学研究所生化研究室,河北石家庄050021 [2]河北省医学科学院附属医院,河北石家庄050021

出　　处：《高血压杂志》2002年第5期454-456,共3页Chinese Journal of Hypertension

摘　　要：目的　为了探讨内源性Na+ K+ ATP酶 (钠泵 )抑制因子水平与高血压发病之间的关系。方法　我们检测了原发性高血压患者 4 8例 ,正常血压者 35例 ;妊娠高血压患者 15例 ,正常孕妇 2 0例采用反相C18预柱处理及酶联免疫吸附法 (ELISA)测定血清中哇巴因样物质 (OLC)和前海葱苷原A样物质 (PLC)水平。结果　原发性高血压患者血清中OLC水平为 132± 2 1pmol L ,PLC水平为 5 .85± 2 .5 9pmol L ,分别明显高于正常人 16 .4± 5 .2pmol L和 0 4 2± 0 2 0pmol L(P <0 0 0 1) ;妊娠高血压患者血清中OLC和PLC水平分别为 34.6± 11.12pmol L和 10 2± 5 0 5pmol L ,明显高于正常孕妇 3.8± 0 86pmol L和 1.2± 0 14pmol L(P <0 0 0 1)。结论　证明高血压患者血液中的内源性钠泵抑制因子水平升高可能是高血压发病的重要因素之一。Aim To study the endogenous sodium pump inhibitor in patients with essential hypertension and pregnancy hypertension. Methods Forty\|eight hypertension patients, 35 normotensive subjects, 15 pregnancy hypertension and 20 normotensive pregnancy were recruited. All antihypertensive agents were stopped 2 weeks before the study. The serum ouabain like compound (OLC) and proscillaridin A like compound (PLC) were measured by a specific enzyme linked immunosorbent assay (ELISA). Results OLC and PLC in hypertension patients(OLC:132±21 pmol/L,PLC:5.85±2.59 pmol/L) were significantly greater than that in normotensive subjects (OLC:16.4±5.2 pmol/L,PLC:0 42±0 20 pmol/L, P< 0 001); Higher concentration of OLC and PLC were found in the pregnancy patients(34.6±11.12 pmol/L and 10 2±5 05 pmol/L ) than normotensive pregnancy (3.8±0 86 pmol/L and 1.2±0 14 pmol/L, P< 0 001). Conclusion This both studies showr that higher concentration of the ouabain immunoreactive and proscillaridin A immunoreactive materials in blood was found in essential hypertension and pregnancy hypertension.

关 键 词：原发性高血压 妊娠高血压 血清 钠泵抑制因子 免疫活性物 测定 

分 类 号：R544.1[医药卫生—心血管疾病] R714.246[医药卫生—内科学]