基因芯片检测乙型肝炎病毒YMDD变异的临床应用  

Clinical usefulness of genomic chip for detection of HBV YMDD mutation in patients with chronic hepatitis B

作　　者：计焱焱 徐蓓 姚光弼 倪晓龙 吴亦栋 马宁 

机构地区：[1]上海静安区中心医院临床免疫研究中心,200040 [2]上海博华基因芯片技术有限公司

出　　处：《肝脏》2002年第3期171-173,共3页Chinese Hepatology

摘　　要：目的　评估基因芯片法检测乙型肝炎病毒 (HBV)YMDD变异的临床应用。方法　用基因芯片法检测 60份乙型肝炎病人的血清 ,其中 2 0份同时用基因测序法和快速PCR法检测 ,用Ridit方法分析检测结果。结果　拉米夫定治疗 5 2周的 2 0个标本中 ,用基因芯片法检测出 14份有YMDD变异株 ,与快速PCR法检出结果一致 ,测序法仅检出9例有变异株。Objective To evaluate the clinical usefulness of genomic chip for detection of HBV YMDD mutation. Methods 60 blood specimens of chronic hepatitis B patients treated with lamivudine were examined by genomic chip method for YMDD mutant strains which were compared with those by sequencing analysis and rapid PCR method. Results YMDD mutation strains were detected in 14/20 specimens from 20 patients treated with lamivudine for 52 weeks. In 14 specimens mutations were also demonstrated by rapid PCR method. Sequencing analysis method only found 9 mutation strains.Conclusion Genomic chip was a usefal method for detection of HBV mutation in clinical.

关 键 词：乙型肝炎病毒 YMDD 临床应用 基因芯片 乙型肝炎 YMDD变异 多聚酶活性区核苷酸序列 

分 类 号：R346[医药卫生—基础医学]