光叶丁公藤酚性成分研究  

Phenolic constituents from Erycibe schmidtii

作　　者：周岳[1] 曾婷[1] 李丽梅[1] 

机构地区：[1]成都医学院,四川成都610500

出　　处：《中草药》2016年第9期1496-1500,共5页Chinese Traditional and Herbal Drugs

基　　金：四川省杰出青年学术技术带头人培育计划(2014JQ0049)

摘　　要：目的研究光叶丁公藤Erycibe schmidtii藤茎的化学成分。方法利用正、反相硅胶色谱柱、Sephadex LH-20色谱及半制备高效液相色谱等方法分离纯化,通过核磁共振波谱分析方法鉴定结构。利用NO相对生成率法对量较多的化合物进行抗炎活性检测。结果从光叶丁公藤藤茎95%乙醇提取物中分离得到12个化合物,分别鉴定为咖啡酸(1)、N-反式-对羟基苯乙基阿魏酰胺(2)、绿原酸(3)、绿原酸甲酯(4)、4-O-咖啡酰基奎宁酸(5)、4-O-咖啡酰基奎宁酸甲酯(6)、4,5-O-双咖啡酰基奎宁酸(7)、4,5-O-双咖啡酰基奎宁酸甲酯(8)、3,5-O-双咖啡酰基奎宁酸(9)、3,5-O-双咖啡酰基奎宁酸甲酯(10)、3,4-O-双咖啡酰基奎宁酸(11)、3,4-O-双咖啡酰基奎宁酸甲酯(12)。化合物1和2对RAW 264.7细胞释放NO显示出一定的抑制活性。结论化合物5~7、9、11、12为首次从该属植物中分离得到,化合物1、2、4~12为首次从该植物中分离得到。化合物1和2有一定抗炎活性。Objective To investigate the chemical constituents of Erycibe schmidtii.Methods The compounds were separated and purified by silica gel and Sephadex LH-20 column chromatography,and semipreparative HPLC.Their structures were determined by nuclear magnetic resonance(NMR) spectroscopy analysis method.The anti-inflammatory activity of some compounds was tested by relative NO productivity.Results Twelve compounds were isolated and identified as caffeic acid(1),N-trans-p-hydroxyphenethyl ferolamine(2),chlorogenic acid(3),chlorogenic acid methyl ester(4),4-O-caffeoylquinic acid(5),4-O-caffeoylquinic acid methyl ester(6),4,5-di-O-caffeoylquinic acid(7),4,5-di-O-caffeoylquinic acid methyl ester(8),3,5-di-O-caffeoylquinic acid(9),3,5-di-O-caffeoylquinic acid methyl ester(10),3,4-di-O-caffeoylquinic acid(11),and 3,4-di-O-caffeoylquinic acid methyl ester(12).Compounds 1 and 2 showed some inhibitory activity for RAW cells 264.7 releasing NO.Conclusion Compounds 5—7,9,11,and 12 are obtained from the plants of Erycibe Roxb.for the first time,while compounds 1,2,and 4—12 are separated from this plant for the first time.Compounds 1 and 2 exhibit certain anti-inflammatory activity.

关 键 词：光叶丁公藤 酚性成分 4-O-咖啡酰基奎宁酸 4 5-O-双咖啡酰基奎宁酸 3 5-O-双咖啡酰基奎宁酸 抗炎活性 NO 

分 类 号：R284.1[医药卫生—中药学]