蜜蜂中肠一株耐氯霉素细菌的筛选与鉴定  

Screening and identification of a chloramphenicol-resistant bacteria strain from the honeybee midgut

作　　者：陈大福[1] 陈梅强 李江红[1] 梁勤[1] 

机构地区：[1]福建农林大学蜂学学院,福州350002

出　　处：《中国蜂业》2012年第Z4期39-43,共5页Apiculture of China

基　　金：福建省自然科学基金项目(2011J01068);国家蜂产业技术体系建设专项资金(CARS-45-KXJ7)资助

摘　　要：细菌的16S核糖体RNA(ribosome RNA,rRNA)基因以其在进化上的特征性序列,现已被广泛用于细菌分类和鉴定的分子指标。本研究用终浓度为20μg/mL的氯霉素从蜜蜂中肠筛选得到耐氯霉素的菌株,经对其16S rRNA基因片段克隆测序,再与NCBI数据库中已知细菌的16S rRNA基因片段序列相比对,结果表明经氯霉素筛选菌株的16S rRNA基因片段序列与克雷伯氏菌(Klebsiella pneumoniae)的相关序列相似度为100%,因此可将该菌株初步鉴定为克雷伯氏菌。该菌株在供试蜜蜂中肠内对氯霉素具有一定的耐药性。The 16S ribosomes RNA gene sequences as a molecular markers have been extensively applied to the classification and identification of bacteria.In this study, a bacteria strain was screened from Italian honeybee midgut by 20 μg/mL chloramphenicol LB culture medium, and the 16S rRNA gene sequence of the strain was amplified and compared with those of other bacteria in GenBank.The 16S rRNA gene sequence analysis showed the strain was 100% identity with that of Klebsiella pneumoniae in GenBank.In summary, the bacteria strain was preliminarily identified as Klebsiella pneumoniae and it was resistant to chloramphenicol in a certain degree.

关 键 词：意大利蜜蜂 中肠 细菌 氯霉素 鉴定 

分 类 号：S891[农业科学—特种经济动物饲养]