Detection of Neisseria Gonorrhoeae from Urine with Ligase Chain Reaction  

连接酶链反应检测尿液中的淋病双球菌(英文)

作　　者：曹经江[1] 郑和义[1] 胡维[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院皮肤科,北京100730

出　　处：《Chinese Journal of Sexually Transmitted Infections》2003年第2期33-36,68,共6页中华性传播感染杂志（英文版）

摘　　要：Objective: To evaluate the value of ligase chainreaction(LCR) in the diagnosis of diplococcusgonorrhoeae in urine. Methods: LCR detection of the urine for Neisseriagonorrhoeae and bacteria culture of discharge was per-formed simultaneously to 276 patients with urethritisor cervicitis seeking treatment in sex transmitted dis-eases (STDs) outpatient clinic. For specimens withdiscordant results, polymerase chain reaction wasconducted. The purpose was to detect the respectivesensitivity and specificity of bacteria culture and LCR. Results: 24 of 276(8.7%) patients had positive LCRresults and 21 of 276(7.6%) were positive for culture.5 specimens had discordant results from LCR andbacteria culture. The sensitivity and specificity of LCRin the diagnosis of gonorrhoeae were 92.3% and100% respectively. Conclusion: This study showed that LCR had ahigher sensitivity and specificity for the diagnosis ofgonorrhoeae from urine.目的评价连接酶链反应(LCR)技术对尿液中淋病双球菌诊断的意义。方法对在STD门诊就诊的276例尿道/宫颈炎患者进行分泌物细菌培养及尿液淋球菌LCR检测,对两项结果不符合的标本进行聚合酶链反应(PCR)检测,确定细菌培养、LCR法检测尿液中淋球菌的敏感性、特异性。结果 276例患者中24例LCR检测阳性(8.7%),21例培养阳性(7.6%)。5例两项结果不符合者进行聚合酶链反应检测。结果表明LCR的敏感性及特异性分别为92.3%,100%。结论 LCR分析法检测尿液中的淋球菌具有较高的敏感性及特异性。

关 键 词：neisseria gonorrhocae ligatse chain reaction URINE 

分 类 号：R446.5[医药卫生—诊断学]