AFLP fingerprinting of Chinese epidemic strains of Puccinia striiformis f. sp. tritici  

作　　者：郑文明 刘峰 康振生 陈受宜 李振岐 吴立人 

机构地区：[1]Department of Plant Protection, Northwestern Agricultural University, Yangling 712100, China Bioengineering College, Henan Agricultural University, Zhengzhou 450002, China [2]Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China [3]Department of Plant Protection, Northwestern Agricultural University, Yangling 712100, China [4]Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing 100094, China

出　　处：《Progress in Natural Science:Materials International》2001年第8期29-35,共7页自然科学进展·国际材料（英文版）

基　　金：Project supported by the National Natural Science Foundation of China (Grant No. 39770486) and National'973'Project (Grant No. G200016201).

摘　　要：Amplified fragment length polymorphism (AFLP) was used to fingerprint the epidemic strains CY25, CY27, CY28, CY29, CY30, CY31, Hy3, Hy7, Syl3 and a mutant strain WV-4 of P. striiformis f. sp. tritici, the pathogen of wheat stripe rust. The results showed that (i) genetic diversity existed in the pathogen populations, and based on it a dendrogram of these strains was constructed by unweighted pair-group mean average to demonstrate the relationships of the tested strains; (ii) no significant correlation between virulence of the pathogens and the polymorphism of DNA fingerprints was found; (iii) AFLP fingerprints showed higher polymorphism than that of the virulence variation; (iv) several new pathorypes identified might evolve independently of the reference strains identified before.Amplified fragment length polymorphism (AFLP) was used to fingerprint the epidemic strains CY25, CY27, CY28, CY29, CY30, CY31, Hy3, Hy7, Syl3 and a mutant strain WV-4 of P. striiformis f. sp. tritici, the pathogen of wheat stripe rust. The results showed that (i) genetic diversity existed in the pathogen populations, and based on it a dendrogram of these strains was constructed by unweighted pair-group mean average to demonstrate the relationships of the tested strains; (ii) no significant correlation between virulence of the pathogens and the polymorphism of DNA fingerprints was found; (iii) AFLP fingerprints showed higher polymorphism than that of the virulence variation; (iv) several new pathorypes identified might evolve independently of the reference strains identified before.

关 键 词：PUCCINIA STRIIFORMIS AFLP DNA fingerprinting CLUSTER analysis EVOLUTION relationship. 

分 类 号：Q987[生物学—遗传学]