CSA对SfaMNPV诱导SL-1细胞凋亡的影响  

Influence of CSA on Apoptosis Induced by SfaMNPV In SL-1 Cells

作　　者：袁璞[1] 彭建新[1] 郭素英[1] 

机构地区：[1]华中师范大学昆虫学研究所,武汉430079

出　　处：《华中师范大学研究生学报》2008年第3期117-120,共4页Central China Normal University Journal of Postgraduates

基　　金：国家自然科学基金资助项目(30370056)。

摘　　要：检测线粒体膜通透性转运孔(MPT)的开放抑制剂环孢菌素A(CSA)对芹菜夜蛾核型多角体病毒(Syngrapha falcifera multiplenuclear polyhedrosis virus,SfaMNPV)诱导诱导斜纹夜蛾(Spodoptera litura Cells,SL-1)细胞凋亡的影响。用不同浓度CSA和SfaMNPV单独或联合处理SL-1细胞,通过细胞形态学观察、DNA凝胶电泳法、PI染色流式细胞仪鉴定细胞凋亡,应用罗丹明123(Rh123)染色流式细胞仪测定线粒体跨膜电位(ΔΨm)。CSA明显抑制了SfaMNPV诱导的SL-1细胞凋亡,CSA还抑制了SfaMNPV诱导的SL-1细胞线粒体膜电位(ΔΨm)下降。CSA抑制了SfaMNPV诱导SL-1细胞的ΔΨm下降,可能是亲环素-D(CyP-D)与CSA的结合导致MPT的关闭,从而抑制细胞凋亡。To investigate the effects of mitochondrial membrane permeability transition pore MPT-closed agent CSA on SfaMNPV induced apoptosis in SL-1 cell line. SL-1 cells were treated with SfaMNPV alone or in combination with CSA in different concentrations. Cell apoptosis was assessed by the morphological observation , DNA electrophoresis and flow cytometry. The mitochondrial transmembrane potentials (ΔΨm) were detected by flow cytometry according to the intensity of rhodamine 123 uptake in cells. CSA prevented SL-1 cells treated by SfaMNPV from apoptosis. CSA significantly restrained mitochondrial membrane potential of apoptotic SL-1 cells from degression. CSA restraining ΔΨm from degression possibly involves with CSA binding to CyP-D and CSA prevented SL-1 cells from apoptosis.

关 键 词：环孢菌素A 芹菜夜蛾核型多角体病毒 斜纹夜蛾细胞系 细胞凋亡 线粒体 

分 类 号：S476[农业科学—农业昆虫与害虫防治]