梯度蔗糖诱导黄皮胚轴脱水耐性后超低温保存  

Cryopreservation of Wampee(Clausena lansium) Axes after Induction of Desiccation-tolerance by Gradient Sucrose

作　　者：黄雪梅[1] 傅家瑞[2] 宋松泉[3] 

机构地区：[1]华南农业大学园艺学院,广东广州51064 [2]中山大学生命科学院,广东广州510275 [3]中国科学院西双版纳热带植物园

出　　处：《中山大学学报（自然科学版）》2003年第S2期231-235,共5页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广东省自然科学基金资助项目(980360)

摘　　要：梯度蔗糖预培养能增强黄皮胚轴的脱水耐性,使胚轴含水量下降至17 6%仍保持活力,在含NAA和IAA的WPM培养基上可诱导植株再生。黄皮胚轴脱水至18%后分别采用直接冻存法、抗冻剂冻存法、微滴冻法和玻璃化法超低温保存都不能复活。玻璃化冻存后的胚芽超微结构基本完整,若能继续优化冻存和培养条件,有希望获得存活的胚芽及其再生植株。Pretreatment with gradient sucrose significantly enhanced desiccation-tolerance of wampee axes which could survival under the moisture of 17.6%. Plantlets could be regenerated from the dried axes on woody plant medium contented hormones of α-napthaleneacetic acid and indole-3-butyric acid. These axes with moisture content of 18% could not survival liquid nitrogen(-196 ℃) even if by using cryoprotectants, droplet freezing and vitrification. However, substructure of plumule were almost intact during cryopreservation of the axes by vitrificating. The present technique of cryopreservation and reculture should by improved for the successful cryopreservation of wampee axes.

关 键 词：黄皮胚轴 梯度蔗糖 脱水耐性诱导 超低温保存 

分 类 号：S666.6[农业科学—果树学]