渗透胁迫诱导的小麦叶片细胞程序性死亡  

Programmed Cell Death Induced by Osmotic Stress in Wheat Leaves

作　　者：林久生[1] 王根轩[1] 

机构地区：[1]兰州大学干旱农业生态国家重点实验室,兰州730000

出　　处：《植物生理与分子生物学学报》2001年第3期221-225,共5页Journal Of Plant Physiology and Molecular Biology

基　　金：国家自然科学基金 ( 39770 44 7);国家重点基础研究专项经费(G19990 1170 5 )资助

摘　　要：用 2 0 %PEG60 0 0 ( - 0 .63MPa)溶液对小麦(Triticumaestivum)根系进行渗透胁迫 ,在DNA琼脂糖凝胶电泳图谱上观察到明显的梯状DNA条带 ,表明PEG处理诱发了DNA核小体间的断裂 ,从而表现出典型的细胞程序性死亡的生化特征 ;末端脱氧核糖核酸转移酶介导的 3′ OH末端标记法 (terminaldeoxynu cleotidyltransferase (TdT) mediateddUTPnickendlabel ing ,TUNEL)检测出现阳性结果。这些结果表明在PEG诱导小麦叶片死亡过程中 ,有一个阶段存在明显的细胞程序性死亡过程。结果同时表明 ,在小麦叶片处于程序性死亡时期 ,蛋白质含量、叶绿素含量的下降和电解质泄漏率的增加都比处于坏死时期的慢。PEG solution (-0.63 MPa) was used as osmotic stress to induce cell death in wheat (Triticum aestivum) leaves. The DNA ladder, which is the typical character of programmed cell death (PCD), was observed in the leaves after being treated for 3 to 5 days (Fig.1). Under the same conditions, positive reaction to the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) procedure also appeared (Fig.2). These results indicated that osmotic stress could induce PCD in wheat leaves. During PCD, the decrease in chlorophyll and protein contents and the increase in electrolyte leakage were all slower than those during necrosis (Fig.3).

关 键 词：渗透胁迫 小麦叶片 细胞程序性死亡 DNA梯状条带 TUNEL 

分 类 号：S435.12[农业科学—农业昆虫与害虫防治]