甘蓝型油菜BcNA1基因启动子在转基因烟草中对GUS基因表达的调控  

Expression of GUS Gene in Transgenic Tobacco Regulated by BcNA1 Promoter from Brassica napus

作　　者：石东乔[1] 周奕华[1] 万里红[1] 刘桂珍[1] 胡赞民[1] 陈正华[1] 

机构地区：[1]中国科学院遗传研究所,北京100101

出　　处：《植物生理与分子生物学学报》2001年第4期313-320,共8页Journal Of Plant Physiology and Molecular Biology

基　　金：国家自然科学基金资助 (3 9770 465 )

摘　　要：通过PCR扩增 ,从甘蓝型油菜 (Brassicanapus)品种H16 5中克隆了种子贮藏蛋白基因BcNA1的启动子 ,将此启动子与GUS基因相连构建了植物表达载体 ,利用农杆菌介导法将其导入烟草。对转基因烟草GUS基因检测分析表明 ,BcNA1基因启动子能特异地启动GUS基因在种子中的表达 ;而且GUS酶的活性随着转基因烟草种子的发育而变化 。The upstream regulatory region of a seed specific gene was separated from Brassica napus cv. H165 genomic DNA by polymerase chain reaction. Plant expression vector was constructed by ligating this promoter with GUS gene and transferred into tobacco. GUS analysis made on the transgenic plants indicated that the promoter could drive the expression of GUS gene seed specifically and the activity of GUS changed with the development of seeds. The results of GUS assay also suggested that the genetic transmission of the BcNA1 promoter conforms to Mendelian laws.

关 键 词：油菜 BcNA1启动子 植物表达载体 农杆菌介导 烟草 

分 类 号：Q74[生物学—分子生物学]