观察HLA-DQ2、饮食暴露和乳糜泻对儿童谷蛋白抗体形成中的诱导作用  

Effect of HLA DQ2,dietary exposure and coeliac disease on the development of antibody response to gliadin in children

作　　者：Ludvigsson J. Eylert M. Ilonen J. O.Vaarala 沙焕臣 

机构地区：[1]Department of Molecular and Clinical Medicine,Faculty of Health Sciences,Linkping University,,Sweden

出　　处：《世界核心医学期刊文摘（胃肠病学分册）》2006年第12期54-55,共2页Core Journals in Gastroenterology

摘　　要：Objective.To study the effect of HLA DQ2,dietary history and development of coeliac disease(CD) on the induction of antibody response to wheat gliadin and cow’s milk,beta-lactoglobulin between 1 and 2.5 years of age in children who developed CD and in healthy children.Material and methods.Infants participating in a birth cohort study(the ABIS study) in Sweden were studied.Thirty-nine children developed CD(cases) ,confirmed through biopsy,during follow-up until 2.5-5 years of age.A total of 181 healthy control children were matched for duration of exclusive breast-feeding,birth-weight,gender,maternal smoking and season of birth.IgG and IgA antigliadin and anti-beta-lactoglobulin antibodies were measured using enzyme immunoassay(EIA) .The effects of HLA-risk genotypes,DQ2 and DQ8,on CD were also considered.Results.Children who developed CD had higher IgG and IgA antigliadin and anti-beta-lactoglobulin antibody levels at 1 year of age than controls(all comparisons:p < 0.001) .Similar differences were seen between cases with as yet undiagnosed CD by 1 year of age and controls,and also when cases were compared with HLA-matched controls.Higher levels of IgG and IgA antibodies to beta-lactoglobu-lin(p = 0.003;p = 0.001) ,but not to gliadin,were found in treated cases versus controls at 2.5 years of age.HLA-DQ2-positive healthy children had lower levels of IgG and IgA antigliadin antibodies than HLADQ2 negative controls at 1 year of age(p = 0.004;p = 0.012) .Conc-lusions.Enhanced humoral response emerging not only to gliadin,but also to other food antigens seems to be primarily associated with CD.Poor induction of antibody response to wheat gliadin in healthy children with the HLA-DQ2 risk molecule could at least partly explain the genetic predisposition to gluten intolerance and CD.Objective.To study the effect of HLA DQ2,dietary history and development of coeliac disease(CD) on the induction of antibody response to wheat gliadin and cow's milk,beta-lactoglobulin between 1 and 2.5 years of age in children who developed CD and in healthy children.Material and methods.Infants participating in a birth cohort study(the ABIS study) in Sweden were studied.Thirty-nine children developed CD(cases) ,confirmed through biopsy,during follow-up until 2.5-5 years of age.A total of 181 healthy control children were matched for duration of exclusive breast-feeding,birth-weight,gender,maternal smoking and season of birth.IgG and IgA antigliadin and anti-beta-lactoglobulin antibodies were measured using enzyme immunoassay(EIA) .The effects of HLA-risk genotypes,DQ2 and DQ8,on CD were also considered.Results.Children who developed CD had higher IgG and IgA antigliadin and anti-beta-lactoglobulin antibody levels at 1 year of age than controls(all comparisons:p < 0.001) .Similar differences were seen between cases with as yet undiagnosed CD by 1 year of age and controls,and also when cases were compared with HLA-matched controls.Higher levels of IgG and IgA antibodies to beta-lactoglobu-lin(p = 0.003;p = 0.001) ,but not to gliadin,were found in treated cases versus controls at 2.5 years of age.HLA-DQ2-positive healthy children had lower levels of IgG and IgA antigliadin antibodies than HLADQ2 negative controls at 1 year of age(p = 0.004;p = 0.012) .Conc-lusions.Enhanced humoral response emerging not only to gliadin,but also to other food antigens seems to be primarily associated with CD.Poor induction of antibody response to wheat gliadin in healthy children with the HLA-DQ2 risk molecule could at least partly explain the genetic predisposition to gluten intolerance and CD.

关 键 词：HLA-DQ2 抗体形成 乳糜泻 谷蛋白 体液免疫应答 Β-乳球蛋白 蛋白抗体 组织学检查 抗体反应 

分 类 号：R725.7[医药卫生—儿科]