天佛参逆转K_(562)/ADM细胞株耐药性的作用及机制探讨  

Reversal effects and mechanism study of Tianfoshen on drugresistance of human erythrocyte leukaemic cell strain K_(562)/ADM

作　　者：杨国武[1] 党琦[2] 李新民[2] 

机构地区：[1]中国人民解放军第135医院肿瘤科,山东省胶州市266300 [2]陕西中医学院肿瘤教研室,咸阳市712083

出　　处：《现代中西医结合杂志》2002年第19期1865-1867,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的 :研究复方天佛参口服液 (TFS)逆转人红白血病耐药细胞株K56 2 /ADM耐药性的作用 ,探讨该药对K56 2 /ADM细胞膜P -糖蛋白 (P -gp)的功能及表达的影响。方法 :体外培养K56 2 /ADM细胞 ,分别用MTT法、流式细胞仪、免疫组化法等方法观察TFS对K56 2 /ADM细胞生长的抑制作用 ,检测K56 2 /ADM细胞膜上P -gp的功能及表达情况。结果 :TFS在非毒性浓度以下能逆转细胞耐药 ,与ADM合用能抑制K56 2 /ADM细胞生长 (P <0 .0 0 5 ) ;K56 2 /ADM细胞内药物浓度升高 ;K56 2 /ADM细胞上P -gp的高表达 ,经TFS作用后其表达下降 ,且 48h后完全消失 ,呈时间依赖性。结论 :TFS能逆转K56 2 /ADM耐药性 ,其机制与抑制K56 2 /ADM细胞膜上P -gp高表达有关。Objective: It is to study Tianfoshen's (TFS) reversal effects on drugresistant of human erythrocyte leukaemia cell strain K 562 /ADR and discuss its effect to P?glucoprotein of K 562 /ADM cell membrane. Methods: K 562 /ADM cells were cultured in vitro and the restrain effects of TFS on K 562 /ADM cell growth were determined with MTT corlorimetric technique, flow?cytometry and immunochemical assay etc. The function and expression of P?glucoprotein in K 562 /ADM cell membrane were observed. Results: With non?cytotoxicity concentration, TFS reserved the multudrug resistance of K 562 /ADM cell membrane, and which combined with ADM had the potential to restrain the growth of K 562 /ADM cells ( P <0.005). Fluorescent intensity of DNR in K 562 /ADM cells significantly increased. P?glucoprotein expressions of K 562 /ADM cells decreased with TFS and completely disappeared after 48 hours treated with TFS. Conclusion: TFS can reverse the drugresistance of K 562 /ADM cells and the mechanism is related to high expression of P?glucoprotein of K 562 /ADM cell membrane.

关 键 词：天佛参口服液 多药耐药 P-糖蛋白 逆转 

分 类 号：R286.91[医药卫生—中药学]